ELISA/assay

Rat MMP-12 (Matrix Metalloproteinase 12) ELISA Kit

MBS2514853

48-Strip-Wells

410 USD

ELISA Kit

Rat MMP-12 (Matrix Metalloproteinase 12) ELISA Kit

MMP-12

macrophage metalloelastase preproprotein; Macrophage metalloelastase; macrophage metalloelastase; matrix metalloproteinase 12 (macrophage elastase); matrix metallopeptidase 12 (macrophage elastase); Macrophage elastase; ME; hME; Matrix metalloproteinase-12; MMP-12

MMP-12

MMP12; MMP12; ME; HME; MME; MMP-12; HME; MME; ME; hME; MMP-12

MMP12_HUMAN

Rat

470

This kit recognizes natural and recombinant Rat MMP-12. No significant cross-reactivity or interference between Rat MMP-12 and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 78.125-5000pg/mL. Sensitivity: 46.875pg/mL

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat MMP-12 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MMP-12. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MMP-12 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MMP-12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of MMP-12. You can calculate the concentration of MMP-12 in the samples by comparing the OD of the samples to the standard curve.

73858572

NP_002417.2

NM_002426.4

P39900

54,002 Da

Collagen Degradation Pathway (730309); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); Matrix Metalloproteinases Pathway (198900)

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]

MMP12: May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1 site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. Belongs to the peptidase M10A family. Protein type: Secreted, signal peptide; EC 3.4.24.65; Secreted; Protease. Chromosomal Location of Human Ortholog: 11q22.3. Cellular Component: proteinaceous extracellular matrix; extracellular region. Molecular Function: zinc ion binding; metalloendopeptidase activity; endopeptidase activity; calcium ion binding. Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; positive regulation of epithelial cell proliferation involved in wound healing; proteolysis; wound healing, spreading of epidermal cells

48-Strip-Wells

410 USD

96-Strip-Wells

490