catalog number :
MBS2514783
products type :
ELISA Kit
products full name :
Porcine EGF (Epidermal Growth Factor) ELISA Kit
products short name :
[EGF]
other names :
[epidermal growth factor, partial; Epidermal growth factor; Epidermal growth factor]
products gene name :
[EGF]
other gene names :
[epidermal growth factor/ EGF]
uniprot entry name :
Q28867_HORSE
specificity :
This kit recognizes Porcine Cyt-C in samples. No significant cross-reactivity or interference between Porcine Cyt-C and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.31-20ng/mL. Sensitivity: 0.19ng/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Porcine Cyt-C were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Porcine Cyt-C were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine Cyt-C concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine Cyt-C. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine Cyt-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine Cyt-C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Porcine Cyt-C. You can calculate the concentration of Porcine Cyt-C in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AAB32226.1
ncbi mol weight :
9,879 Da
size5 :
10x96-Strip-Wells