Mouse PAPP-A (pregnancy associated plasma protein A) ELISA Kit | MyBioSource MBS2514768 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse PAPP-A (pregnancy associated plasma protein A) ELISA Kit

catalog :

MBS2514768

quantity :

24-Strip-Wells

price :

215 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2514768

products type :

ELISA Kit

products full name :

Mouse PAPP-A (pregnancy associated plasma protein A) ELISA Kit

products short name :

[PAPP-A]

other names :

[pappalysin-1 preproprotein; Pappalysin-1; pappalysin-1; IGF-dependent IGFBP-4 protease; aspecific BCL2 ARE-binding protein 2; differentially placenta 1 expressed protein; insulin-like growth factor-dependent IGF binding protein-4 protease; insulin-like growth factor-dependent IGF-binding protein 4 protease; pregnacy-associated plasma protein A; pregnancy-associated plasma protein A, pappalysin 1; Insulin-like growth factor-dependent IGF-binding protein 4 protease; IGF-dependent IGFBP-4 protease; IGFBP-4ase; Pregnancy-associated plasma protein A; PAPP-A]

products gene name :

[PAPP-A]

other gene names :

[PAPPA; PAPPA; PAPA; DIPLA1; PAPP-A; PAPPA1; ASBABP2; IGFBP-4ase; IGF-dependent IGFBP-4 protease; IGFBP-4ase; PAPP-A]

uniprot entry name :

PAPP1_HUMAN

reactivity :

Mouse

sequence length :

1627

specificity :

This kit recognizes Mouse PAF in samples. No significant cross-reactivity or interference between Mouse PAF and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.78-50ng/mL. Sensitivity: 0.47ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse PAF were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse PAF were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse PAF concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PAF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PAF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PAF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse PAF. You can calculate the concentration of Mouse PAF in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

38045915

ncbi acc num :

NP_002572.2

ncbi gb acc num :

NM_002581.3

uniprot acc num :

Q13219

ncbi mol weight :

180,973 Da

ncbi pathways :

Metabolism Of Proteins Pathway (106230); Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway (105908)

ncbi summary :

This gene encodes a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs). It is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses. [provided by RefSeq, Jul 2008]

uniprot summary :

PAPPA: Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF. Belongs to the peptidase M43B family. Protein type: Protease; EC 3.4.24.79; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 9q33.2. Cellular Component: membrane; extracellular region. Molecular Function: metallopeptidase activity; zinc ion binding; metalloendopeptidase activity. Biological Process: cellular protein metabolic process; female pregnancy; cell differentiation; proteolysis

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590

more info or order :

MyBioSource product webpage