catalog number :
MBS2514724
products type :
ELISA Kit
products full name :
Rat ICTP (Cross-linked C-Telopeptide of Type I Collagen) ELISA Kit
products short name :
ICTP
products gene name :
ICTP
specificity :
This kit recognizes natural and recombinant Rat ICTP. No significant cross-reactivity or interference between Rat ICTP and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: 0.188ng/mL
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat ICTP concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ICTP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ICTP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ICTP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ICTP. You can calculate the concentration of ICTP in the samples by comparing the OD of the samples to the standard curve.