catalog number :
MBS2514604
products type :
ELISA Kit
products full name :
Porcine MIP-1beta(Macrophage Inflammatory Protein 1 Beta)ELISA Kit
products short name :
MIP-1beta
other names :
MIP-1beta; C-C motif chemokine 4 homolog; C-C motif chemokine 4 homolog; MIP-1-beta; chemokine-like ligand 1; macrophage inflammatory protein 1-beta homolog; small-inducible cytokine A4 homolog; Macrophage inflammatory protein 1-beta homolog; MIP-1-beta; Small-inducible cytokine A4 homolog
products gene name :
MIP-1beta
other gene names :
CCL4; CCL4; SCYA4; MIP-1beta; SCYA4; MIP-1-beta
uniprot entry name :
CCL4_CHICK
specificity :
This assay has high sensitivity and excellent specificity for detection of Porcine MIP-1beta. No significant cross-reactivity or interference between Porcine MIP-1beta and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Porcine MIP-1beta and all the analogues, therefore, cross reaction may still exist.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 31.25--2000pg/mL. Sensitivity: 18.75pg/mL
products description :
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MIP-1beta in Porcine serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine MIP-1beta. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Porcine MIP-1beta and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine MIP-1beta, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Porcine MIP-1beta. You can calculate the concentration of Porcine MIP-1beta in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
ABO77445.1
ncbi mol weight :
9,969 Da
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway (119307); Cytokine-cytokine Receptor Interaction Pathway (460); Cytosolic DNA-sensing Pathway (125152); Cytosolic DNA-sensing Pathway (124833); Salmonella Infection Pathway (375175); Salmonella Infection Pathway (375149); Toll-like Receptor Signaling Pathway (117308); Toll-like Receptor Signaling Pathway (487)