Porcine TNNI2 (Troponin I Type 2, Fast Skeletal) ELISA Kit | MyBioSource MBS2514548 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Porcine TNNI2 (Troponin I Type 2, Fast Skeletal) ELISA Kit

catalog :

MBS2514548

quantity :

24-Strip-Wells

price :

235 USD

product information

catalog number :

MBS2514548

products type :

ELISA Kit

products full name :

Porcine TNNI2 (Troponin I Type 2, Fast Skeletal) ELISA Kit

products short name :

[TNNI2]

other names :

[troponin I, fast skeletal muscle isoform 2; Troponin I, fast skeletal muscle; troponin I, fast skeletal muscle; troponin I fast twitch 2; troponin I, fast-twitch isoform; troponin I, fast-twitch skeletal muscle isoform; troponin I, skeletal, fast; troponin I type 2 (skeletal, fast); Troponin I, fast-twitch isoform]

products gene name :

[TNNI2]

other gene names :

[TNNI2; TNNI2; DA2B; FSSV; fsTnI; AMCD2B]

uniprot entry name :

TNNI2_HUMAN

reactivity :

Porcine

sequence length :

182

specificity :

This kit recognizes natural and recombinant Porcine TNNI2. No significant cross-reactivity or interference between Porcine TNNI2 and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 78.125-5000pg/mL. Sensitivity: Min: 46.875pg/mL; Max: 5000pg/mL

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine TNNI2 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TNNI2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TNNI2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TNNI2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TNNI2. You can calculate the concentration of TNNI2 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

224967106

ncbi acc num :

NP_001139313.1

ncbi gb acc num :

NM_001145841.1

uniprot acc num :

P48788

ncbi mol weight :

21,355 Da

ncbi pathways :

Muscle Contraction Pathway (106261); Striated Muscle Contraction Pathway (198903); Striated Muscle Contraction Pathway (106262)

ncbi summary :

This gene encodes a fast-twitch skeletal muscle protein, a member of the troponin I gene family, and a component of the troponin complex including troponin T, troponin C and troponin I subunits. The troponin complex, along with tropomyosin, is responsible for the calcium-dependent regulation of striated muscle contraction. Mouse studies show that this component is also present in vascular smooth muscle and may play a role in regulation of smooth muscle function. In addition to muscle tissues, this protein is found in corneal epithelium, cartilage where it is an inhibitor of angiogenesis to inhibit tumor growth and metastasis, and mammary gland where it functions as a co-activator of estrogen receptor-related receptor alpha. This protein also suppresses tumor growth in human ovarian carcinoma. Mutations in this gene cause myopathy and distal arthrogryposis type 2B. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]

uniprot summary :

TNNI2: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Defects in TNNI2 are a cause of distal arthrogryposis type 2B (DA2B); also known as arthrogryposis multiplex congenita, distal, type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin. Belongs to the troponin I family. Protein type: Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: troponin complex; cytosol; nucleus. Molecular Function: troponin T binding; protein binding; actin binding. Biological Process: skeletal muscle contraction; regulation of muscle contraction; positive regulation of transcription, DNA-dependent; muscle filament sliding. Disease: Arthrogryposis, Distal, Type 2b

size1 :

24-Strip-Wells

price1 :

235 USD

size2 :

48-Strip-Wells

price2 :

450

size3 :

96-Strip-Wells

price3 :

535

size4 :

5x96-Strip-Wells

price4 :

2255

size5 :

10x96-Strip-Wells

price5 :

3975