catalog number :
MBS2514515
products type :
ELISA Kit
products full name :
Rat CX43 (Connexin 43) ELISA Kit
products short name :
[CX43]
other names :
[Cx43 protein; Gap junction alpha-1 protein; gap junction alpha-1 protein; connexin-43; etID309742.9; gap junction protein, alpha 1; shf; short fin protein; sof; connexin 43; Connexin-43; Cx43; Short fin protein]
products gene name :
[CX43]
other gene names :
[cx43; gja1; gja1; cx43a1; zfCx43.3; cx43; shf; sof; Cx43]
uniprot entry name :
CXA1_DANRE
specificity :
This kit recognizes natural and recombinant Rat CX43. No significant cross-reactivity or interference between Rat CX43 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
products description :
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CX43. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CX43 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CX43, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CX43. You can calculate the concentration of CX43 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AAH49297.1
ncbi mol weight :
39,127 Da
ncbi pathways :
Calcium Regulation In The Cardiac Cell Pathway (198967); EGFR1 Signaling Pathway (198963); Formation Of Annular Gap Junctions Pathway (1114405); Gap Junction Pathway (119270); Gap Junction Pathway (483); Gap Junction Assembly Pathway (1114399); Gap Junction Degradation Pathway (1114404); Gap Junction Trafficking Pathway (1114398); Gap Junction Trafficking And Regulation Pathway (1114397); Membrane Trafficking Pathway (1114388)
uniprot summary :
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Plays an essential role in gap junction communication in the ventricles ().
size5 :
10x96-Strip-Wells