catalog number :
MBS2514493
products type :
ELISA Kit
products full name :
Human F13B (Coagulation Factor XIII B Polypeptide) ELISA Kit
products short name :
[F13B]
other names :
[F13b protein; F13b protein; coagulation factor XIII B chain; coagulation factor XIII, beta subunit; coagulation factor XIII, B polypeptide; F13b protein]
products gene name :
[F13B]
other gene names :
[F13b; F13b]
uniprot entry name :
B1H260_RAT
specificity :
This kit recognizes Human EphA2 in samples. No significant cross-reactivity or interference between Human EphA2 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 78.13-5000pg/mL. Sensitivity: 46.88pg/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human EphA2 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human EphA2 were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human EphA2 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human EphA2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EphA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EphA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human EphA2. You can calculate the concentration of Human EphA2 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AAI60876.1
ncbi mol weight :
75,083 Da
ncbi pathways :
Blood Clotting Cascade Pathway (198493); Complement And Coagulation Cascades Pathway (198461); Complement And Coagulation Cascades Pathway (83465); Complement And Coagulation Cascades Pathway (484); Steroid Biosynthesis Pathway (198481)
uniprot summary :
Enzyme and pathway databasesReactome - a knowledgebase of biological pathways and processes br/> a href='/database/88'>More. /a>ReactomeiR-RNO-140875. Common Pathway of Fibrin Clot Formation.
size5 :
10x96-Strip-Wells