catalog number :
MBS2514473
products type :
ELISA Kit
products full name :
Human PAF (Platelet Activating Factor) ELISA Kit
products short name :
PAF
other names :
PCNA-associated factor isoform 2; PCNA-associated factor; PCNA-associated factor; HCV NS5A-transactivated protein 9; PCNA-associated factor of 15 kDa; hepatitis C virus NS5A-transactivated protein 9; overexpressed in anaplastic thyroid carcinoma 1; KIAA0101; Hepatitis C virus NS5A-transactivated protein 9; HCV NS5A-transactivated protein 9; Overexpressed in anaplastic thyroid carcinoma 1; OEATC-1; PCNA-associated factor of 15 kDa; PAF15; p15PAF
other gene names :
KIAA0101; KIAA0101; L5; PAF; OEATC; PAF15; OEATC1; p15PAF; NS5ATP9; OEATC-1; p15/PAF; p15(PAF); NS5ATP9; PAF; HCV NS5A-transactivated protein 9; OEATC-1; PAF15; p15PAF
uniprot entry name :
PAF15_HUMAN
specificity :
This kit recognizes natural and recombinant Human PAF. No significant cross-reactivity or interference between Human PAF and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/mL. Sensitivity: The minimum detectable dose of Human PAF is 0.188ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
other info2 :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human PAF concentrations in serum, plasma and other biological fluids.
products description :
Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PAF. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PAF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PAF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of PAF. You can calculate the concentration of PAF in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_001025160.1
ncbi gb acc num :
NM_001029989.2
ncbi mol weight :
7,221 Da
uniprot summary :
PAF: May be involved in protection of cells from UV-induced cell death. Protein type: DNA repair, damage. Chromosomal Location of Human Ortholog: 15q22.31. Cellular Component: perinuclear region of cytoplasm; cytoplasm; nucleus. Molecular Function: protein binding; chromatin binding. Biological Process: bypass DNA synthesis; regulation of cell cycle; centrosome organization and biogenesis; DNA replication; response to DNA damage stimulus; response to UV