catalog number :
MBS2514436
products type :
ELISA Kit
products full name :
Monkey HBF (Fetal Hemoglobin) ELISA Kit
products short name :
[HBF]
products gene name :
[HBF]
specificity :
This kit recognizes natural and recombinant Monkey HBF. No significant cross-reactivity or interference between Monkey HBF and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 12.5-800ng/mL. Sensitivity: Min: 7.5ng/mL; Max: 800ng/mL
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Monkey HBF concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HBF. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HBF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HBF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of HBF. You can calculate the concentration of HBF in the samples by comparing the OD of the samples to the standard curve.
size5 :
10x96-Strip-Wells