catalog number :
MBS2514367
products type :
ELISA Kit
products full name :
Rabbit BMP-2 (Bone Morphogenetic Protein 2) ELISA Kit
products short name :
[BMP-2]
other names :
[bone morphogenetic protein 2; Bone morphogenetic protein 2; bone morphogenetic protein 2; BMP-2A; bone morphogenetic protein 2A; bone morphogenetic protein 2; Bone morphogenetic protein 2A; BMP-2A]
products gene name :
[BMP-2]
other gene names :
[BMP2; BMP2; BDA2; BMP2A; BMP2A; BMP-2; BMP-2A]
uniprot entry name :
BMP2_HUMAN
specificity :
This kit recognizes natural and recombinant Rabbit BMP-2. No significant cross-reactivity or interference between Rabbit BMP-2 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL
other info2 :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rabbit BMP-2 concentrations in serum, plasma and other biological fluids.
products description :
. Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to BMP-2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for BMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BMP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of BMP-2. You can calculate the concentration of BMP-2 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AAF21646.1
ncbi mol weight :
44,702 Da
ncbi pathways :
Adipogenesis Pathway (198832); BDNF Signaling Pathway (712093); BMP Signaling Pathway (1084755); BMP Signaling Pathway (1108218); BMP Signalling And Regulation Pathway (198910); Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Elastic Fibre Formation Pathway (730310)
ncbi summary :
The protein encoded by this gene belongs to the transforming growth factor-beta (TGFB) superfamily. The encoded protein acts as a disulfide-linked homodimer and induces bone and cartilage formation. [provided by RefSeq, Jul 2008]
uniprot summary :
Induces cartilage and bone formation (PubMed:3201241). Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4 (PubMed:24362451).
size5 :
10x96-Strip-Wells