ELISA/assay

Human AMPK (Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit

MBS2514316

24-Strip-Wells

215 USD

ELISA Kit

Human AMPK (Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit

[AMPK]

[AMP-activated protein kinase, AMPK; 5'-AMP-activated protein kinase catalytic subunit alpha-2; 5'-AMP-activated protein kinase catalytic subunit alpha-2; 5'-AMP-activated protein kinase, catalytic alpha-2 chain; ACACA kinase; AMP-activated protein kinase alpha-2 subunit variant 2; AMP-activated protein kinase alpha-2 subunit variant 3; AMPK alpha 2; AMPK subunit alpha-2; AMPK-alpha-2 chain; HMGCR kinase; acetyl-CoA carboxylase kinase; hydroxymethylglutaryl-CoA reductase kinase; protein kinase, AMP-activated, alpha 2 catalytic subunit; Acetyl-CoA carboxylase kinase (EC:2.7.11.27); ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase (EC:2.7.11.31); HMGCR kinase]

[AMPK]

[PRKAA2; PRKAA2; AMPK; AMPK2; PRKAA; AMPKa2; AMPK; AMPK2; AMPK subunit alpha-2; ACACA kinase; HMGCR kinase]

AAPK2_HUMAN

Human

552

This kit recognizes Human AICAR in samples. No significant cross-reactivity or interference between Human AICAR and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 12.5-800pg/mL. Sensitivity: 7.5pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human AICAR were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human AICAR were tested on 3 different plates, 20 replicates in each plate.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human AICAR concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AICAR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AICAR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AICAR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human AICAR. You can calculate the concentration of Human AICAR in the samples by comparing the OD of the samples to the standard curve.

786491

AAB32732.1

P54646

62,320 Da

AMPK Inhibits ChREBP Transcriptional Activation Activity Pathway (106102); AMPK Signaling Pathway (198868); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Activation Of PPARGC1A (PGC-1alpha) By Phosphorylation Pathway (1127496); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); BDNF Signaling Pathway (712093); Circadian Rhythm Pathway (83084); Circadian Rhythm Pathway (495)

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq, Jul 2008]

AMPKA2: a catalytic subunit of AMP-activated protein kinase (AMPK). Acts as an energy sensor, playing a key role in regulating cellular energy metabolism. A protein kinase of the CAMKL family whose activation is regulated by the balance between ADP/AMP/ATP, and intracellular Ca(2+) levels. Acts as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5 -ADP and -AMP rise in response to fuel limitation and/or hypoxia. Activates energy-producing pathways and inhibits energy-consuming processes. Restores ATP levels in cells by switching off anabolic and switching on catabolic pathways. Activated primarily by rising ADP levels and not, as previously thought, solely by AMP. AMPK resembles an adenylate charge regulatory system in which anabolic and catabolic pathways are regulated by adenine nucleotide ratios. Acts via direct phosphorylation of metabolic enzymes and transcription regulators. Regulates fatty acid synthesis by phosphorylating acetyl-CoA carboxylase. Regulates cholesterol synthesis by phosphorylating and inactivating hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Activated by at least two distinct upstream kinases: the tumor suppressor LKB1 and CaMKK2. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. AMPK is a heterotrimer of an alpha catalytic subunit (AMPKA1 or -2), a beta (AMPKB1 or -2) and a gamma non-catalytic subunit (AMPKG1, -2 or -3). Different possible combinations of subunits give rise to 12 different holoenzymes. Binding of ADP or AMP to non-catalytic gamma subunit (PRKAG1, -2 or -3) results in allosteric activation. AMPK is activated by antihyperglycemic drug metformin, a drug prescribed to patients with type 2 diabetes: in vivo, metformin seems to mainly inhibit liver gluconeogenesis. However, metformin can be used to activate AMPK in muscle and other cells in culture or ex vivo. Selectively inhibited by compound C (6-[4-(2-Piperidin-1-yl-ethoxy)-phenyl)]-3-pyridin-4-yl-pyyrazolo[1,5-a] pyrimidine. Activated by resveratrol, a natural polyphenol present in red wine, and S17834, a synthetic polyphenol. Salicylate/aspirin directly activates kinase activity. Studies in the mouse suggest that AMPK2 may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. Protein type: Kinase, protein; Autophagy; EC 2.7.11.31; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; EC 2.7.11.1; EC 2.7.11.27; CAMK group; CAMKL family; AMPK subfamily. Chromosomal Location of Human Ortholog: 1p31. Cellular Component: nucleoplasm; cytosol. Molecular Function: AMP-activated protein kinase activity; protein serine/threonine kinase activity; protein binding; metal ion binding; protein serine/threonine/tyrosine kinase activity; [acetyl-CoA carboxylase] kinase activity; histone serine kinase activity; chromatin binding; ATP binding; [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity; protein kinase activity. Biological Process: lipid biosynthetic process; rhythmic process; cellular lipid metabolic process; carnitine shuttle; signal transduction; glucose homeostasis; protein amino acid phosphorylation; cellular response to glucose starvation; cellular response to nutrient levels; regulation of fatty acid biosynthetic process; regulation of transcription, DNA-dependent; response to stress; cell cycle arrest; fatty acid biosynthetic process; positive regulation of autophagy; mitochondrion organization and biogenesis; negative regulation of TOR signaling pathway; Wnt receptor signaling pathway; transcription, DNA-dependent; organelle organization and biogenesis; regulation of circadian rhythm; cholesterol biosynthetic process; fatty acid homeostasis; positive regulation of glycolysis; energy reserve metabolic process; insulin receptor signaling pathway; autophagy; negative regulation of apoptosis

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590