ELISA/assay

Mouse LH (Luteinizing Hormone) ELISA Kit

MBS2514287

24-Strip-Wells

190 USD

ELISA Kit

Mouse LH (Luteinizing Hormone) ELISA Kit

[LH]

[lutropin subunit beta; Lutropin subunit beta; lutropin subunit beta; leutropin; luteinizing hormone subunit beta; lutropin beta chain; luteinizing hormone beta; Luteinizing hormone subunit beta; LH-B; LSH-B; LSH-beta]

[LH]

[Lhb; Lhb; LH; LH-B; LSH-B; LSH-beta; Lutropin beta chain; LH-B; LSH-B; LSH-beta]

LSHB_MOUSE

Mouse

141

This kit recognizes Mouse Klotho in samples. No significant cross-reactivity or interference between Mouse Klotho and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 7.81-500pg/mL. Sensitivity: 4.69pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse Klotho were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse Klotho were tested on 3 different plates, 20 replicates in each plate.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse Klotho concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Klotho. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Klotho and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Klotho, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse Klotho. You can calculate the concentration of Mouse Klotho in the samples by comparing the OD of the samples to the standard curve.

77404413

NP_032523.2

NM_008497.2

O09108

15,028 Da

Androgen Biosynthesis Pathway (1110882); Class A/1 (Rhodopsin-like Receptors) Pathway (1110499); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1111486); Disease Pathway (1111319); G Alpha (s) Signalling Events Pathway (1110529); GPCR Downstream Signaling Pathway (1110528); GPCR Ligand Binding Pathway (1110498); Glycoprotein Hormones Pathway (1110263); GnRH Signaling Pathway (83288); GnRH Signaling Pathway (502)

LHB: Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. Defects in LHB are a cause of hypogonadism LHB-related (HGON-LHB). Hypogonadism is characterized by infertility and pseudohermaphroditism. Belongs to the glycoprotein hormones subunit beta family. Protein type: Secreted, signal peptide; Hormone; Secreted. Cellular Component: extracellular region. Molecular Function: hormone activity. Biological Process: regulation of cGMP metabolic process

24-Strip-Wells

190 USD

48-Strip-Wells

310

96-Strip-Wells

355

5x96-Strip-Wells

1435

10x96-Strip-Wells

2805