Rat SNAP-25(Synaptosome Associated Protein 25)ELISA Kit | MyBioSource MBS2514285 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat SNAP-25(Synaptosome Associated Protein 25)ELISA Kit

catalog :

MBS2514285

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2514285

products type :

ELISA Kit

products full name :

Rat SNAP-25(Synaptosome Associated Protein 25)ELISA Kit

products short name :

SNAP-25

other names :

Synaptosomal-associated protein 25; Synaptosomal-associated protein 25; synaptosomal-associated protein 25; SUP; resistance to inhibitors of cholinesterase 4 homolog; super protein; synaptosomal-associated protein, 25kD; synaptosomal-associated protein, 25kDa; Super protein; SUP; Synaptosomal-associated 25 kDa protein

products gene name :

SNAP-25

other gene names :

SNAP25; SNAP25; RIC4; SEC9; SNAP; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2; SNAP; SNAP-25; SUP

uniprot entry name :

SNP25_HUMAN

reactivity :

Rat

sequence length :

206

specificity :

This assay has high sensitivity and excellent specificity for detection of Rat SNAP-25. No significant cross-reactivity or interference between Rat SNAP-25 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rat SNAP-25 and all the analogues, therefore, cross reaction may still exist.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156--10ng/mL. Sensitivity: 0.094ng/mL

products description :

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SNAP-25 in Rat serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SNAP-25. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat SNAP-25 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SNAP-25, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat SNAP-25. You can calculate the concentration of Rat SNAP-25 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

46397726

ncbi acc num :

P60880.1

ncbi mol weight :

23,336 Da

ncbi pathways :

Acetylcholine Neurotransmitter Release Cycle Pathway (106525); Disease Pathway (530764); Dopamine Neurotransmitter Release Cycle Pathway (106523); Effects Of Botulinum Toxin Pathway (138028); GABA Synthesis, Release, Reuptake And Degradation Pathway (187174); Glutamate Neurotransmitter Release Cycle Pathway (106522); Insulin Signaling Pathway (198845); Insulin Secretion Pathway (777534); Integration Of Energy Metabolism Pathway (106096); Metabolism Pathway (477135)

ncbi summary :

Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

SNAP-25: a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Part of the SNARE core complex containing SNAP25, VAMP2 and syntaxin 1A. Associates with proteins involved in vesicle docking and membrane fusion. Two alternatively spliced isoforms have been described. Protein type: Vesicle. Chromosomal Location of Human Ortholog: 20p12-p11.2. Cellular Component: presynaptic membrane; SNARE complex; neuron projection; growth cone; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; trans-Golgi network; cell junction; cytosol; vesicle. Molecular Function: SNAP receptor activity; protein binding; syntaxin-1 binding; calcium-dependent protein binding. Biological Process: synaptic vesicle fusion to presynaptic membrane; synaptic transmission; synaptic vesicle exocytosis; glutamate secretion; neurotransmitter secretion; energy reserve metabolic process; neurotransmitter uptake; pathogenesis; regulation of insulin secretion; synaptic vesicle priming; synaptic vesicle docking during exocytosis. Disease: Myasthenic Syndrome, Congenital, 18

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage