ELISA/assay

Rat ANG II (Angiotensin II) ELISA Kit

MBS2514276

96-Strip-Wells

445 USD

ELISA Kit

Rat ANG II (Angiotensin II) ELISA Kit

ANG II

angiotensinogen; Angiotensinogen; angiotensinogen; angiotensin II; angiotensinogen (PAT); serpin A8; angiotensinogen (serpin peptidase inhibitor, clade A, member 8); Serpin A8Cleaved into the following 8 chains:Angiotensin-1Alternative name(s):Angiotensin 1-10; Angiotensin I; Ang I

ANG II

Agt; Agt; Ang; PAT; ANRT; AngII; Serpina8; Ang I; Ang II; Ang III; Ang IV

ANGT_RAT

Rat

477

This kit recognizes natural and recombinant Rat ANG II. No significant cross-reactivity or interference between Rat ANG II and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Quantitative Competitive. Sensitivity: 9.375pg/mL

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat ANG II concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with ANG II. During the reaction, ANG II in the sample or standard competes with a fixed amount of ANG II on the solid phase supporter for sites on the Biotinylated Detection Ab specific to ANG II. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ANG II in the samples is then determined by comparing the OD of the samples to the standard curve.

19705570

NP_602308.1

NM_134432.2

P01015

51,982 Da

ACE Inhibitor Pathway (198448); Adipogenesis Pathway (198479); Class A/1 (Rhodopsin-like Receptors) Pathway (1120714); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1122016); Disease Pathway (1121849); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (1120940); G Alpha (i) Signalling Events Pathway (1120745); G Alpha (q) Signalling Events Pathway (1120747); GPCR Downstream Signaling Pathway (1120743); GPCR Ligand Binding Pathway (1120713)

plays a role in regulation of blood pressure [RGD, Feb 2006]

angiotensin: Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-1 (angiotensin 1-10). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2 (angiotensin 1- 8). Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3 (angiotensin 2-8), angiotensin-4 (angiotensin 3-8). Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2. Genetic variations in AGT are a cause of susceptibility to essential hypertension (EHT). Essential hypertension is a condition in which blood pressure is consistently higher than normal with no identifiable cause. Defects in AGT are a cause of renal tubular dysgenesis (RTD). RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Belongs to the serpin family. Protein type: Secreted, signal peptide; Secreted. Cellular Component: extracellular space; cytoplasm. Molecular Function: serine-type endopeptidase inhibitor activity; angiotensin receptor binding; sodium channel regulator activity; hormone activity; type 2 angiotensin receptor binding; type 1 angiotensin receptor binding. Biological Process: positive regulation of catalytic activity; renal system process; extracellular matrix organization and biogenesis; positive regulation of nitric oxide biosynthetic process; establishment of blood-nerve barrier; negative regulation of nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; stress-activated MAPK cascade; positive regulation of multicellular organism growth; female pregnancy; positive regulation of vasodilation; ovarian follicle rupture; regulation of apoptosis; positive regulation of fibroblast proliferation; regulation of systemic arterial blood pressure by circulatory renin-angiotensin; cell surface receptor linked signal transduction; positive regulation of superoxide release; negative regulation of neuron apoptosis; kidney development; angiotensin mediated regulation of renal output; regulation of calcium ion transport; response to muscle activity involved in regulation of muscle adaptation; regulation of norepinephrine secretion; brain renin-angiotensin system; positive regulation of phosphoinositide 3-kinase cascade; negative regulation of tissue remodeling; positive regulation of peptidyl-tyrosine phosphorylation; phospholipase C activation; angiotensin mediated vasoconstriction involved in regulation of systemic arterial blood pressure; regulation of gene expression; regulation of transmission of nerve impulse; smooth muscle cell differentiation; cytokine secretion; positive regulation of blood pressure; regulation of long-term neuronal synaptic plasticity; peristalsis; cell-matrix adhesion; drinking behavior; positive regulation of cellular protein metabolic process; renin-angiotensin regulation of aldosterone production; smooth muscle cell proliferation; excretion; vasodilation; response to salt stress; negative regulation of cell proliferation; fibroblast proliferation; positive regulation of MAPKKK cascade; regulation of blood pressure; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of cell proliferation; angiotensin mediated drinking behavior; artery smooth muscle contraction; aging; vasoconstriction; positive regulation of fatty acid biosynthetic process; blood vessel development; cellular sodium ion homeostasis; renal response to blood flow during renin-angiotensin regulation of systemic arterial blood pressure; activation of NF-kappaB-inducing kinase; positive regulation of organ growth; positive regulation of peptidyl-serine phosphorylation; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; ureteric bud branching; regulation of inflammatory response; hormone metabolic process; response to cold; negative regulation of cell growth; astrocyte activation

96-Strip-Wells

445 USD