catalog number :
MBS2514273
products type :
ELISA Kit
products full name :
Rat TARC (Thymus Activation Regulated Chemokine) ELISA Kit
products short name :
[TARC]
other names :
[thymus and activation-regulated chemokine; C-C motif chemokine; chemokine (C-C motif) ligand 17; chemokine (C-C motif) ligand 17; small inducible cytokine subfamily A17; chemokine (C-C motif) ligand 17]
products gene name :
[TARC]
other gene names :
[Ccl17; Ccl17; Tarc; Abcd-2; Scya17; Scya17l]
uniprot entry name :
Q9WUZ6_MOUSE
specificity :
This kit recognizes natural and recombinant Rat TARC. No significant cross-reactivity or interference between Rat TARC and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL
products description :
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TARC. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TARC and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TARC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TARC. You can calculate the concentration of TARC in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
CAB45256.1
ncbi mol weight :
10,467 Da
ncbi pathways :
Chemokine Receptors Bind Chemokines Pathway (1110501); Chemokine Signaling Pathway (99271); Chemokine Signaling Pathway (672435); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (1110499); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1111486); Disease Pathway (1111319); GPCR Ligand Binding Pathway (1110498)
size5 :
10x96-Strip-Wells