catalog number :
MBS2514255
products type :
ELISA Kit
products full name :
Human iPLA2 (Phospholipidase A2, Calcium Independent) ELISA Kit
products short name :
[iPLA2]
products gene name :
[iPLA2]
specificity :
This kit recognizes natural and recombinant Human iPLA2. No significant cross-reactivity or interference between Human iPLA2 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human iPLA2 concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to iPLA2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for iPLA2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain iPLA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of iPLA2. You can calculate the concentration of iPLA2 in the samples by comparing the OD of the samples to the standard curve.
size5 :
10x96-Strip-Wells