Human PARP4 (Poly ADP Ribose Polymerase 4) ELISA Kit | MyBioSource MBS2514241 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human PARP4 (Poly ADP Ribose Polymerase 4) ELISA Kit

catalog :

MBS2514241

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2514241

products type :

ELISA Kit

products full name :

Human PARP4 (Poly ADP Ribose Polymerase 4) ELISA Kit

products short name :

PARP4

other names :

poly; Poly [ADP-ribose] polymerase 4; poly [ADP-ribose] polymerase 4; poly [ADP-ribose] polymerase 4; 193 kDa vault protein; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)-like 1; ADP-ribosyltransferase diphtheria toxin-like 4; H5 proline-rich; I-alpha-I-related; PARP-related; PARP-related/IalphaI-related H5/proline-rich; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase-like 1; vault poly(ADP-ribose) polymerase; vault protein, 193-kDa; von Willebrand factor A domain containing 5C; poly (ADP-ribose) polymerase family, member 4; 193 kDa vault protein; ADP-ribosyltransferase diphtheria toxin-like 4; ARTD4; PARP-related/IalphaI-related H5/proline-rich; PH5P; Vault poly(ADP-ribose) polymerase; VPARP

products gene name :

PARP4

other gene names :

PARP4; PARP4; PH5P; p193; ARTD4; PARPL; VPARP; VWA5C; PARP-4; VAULT3; ADPRTL1; ADPRTL1; KIAA0177; PARPL; PARP-4; ARTD4; PH5P; VPARP

uniprot entry name :

PARP4_HUMAN

reactivity :

Human

sequence length :

1724

specificity :

This kit recognizes natural and recombinant Human PARP4. No significant cross-reactivity or interference between Human PARP4 and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: The minimum detectable dose of Human PARP4 is 0.094ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).

other info2 :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human PARP4 concentrations in serum, plasma and other biological fluids.

products description :

Priciple of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PARP4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PARP4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PARP4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PARP4. You can calculate the concentration of PARP4 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

112789550

ncbi acc num :

NP_006428.2

ncbi gb acc num :

NM_006437.3

uniprot acc num :

Q9UKK3

ncbi mol weight :

192,595 Da

ncbi pathways :

BER Complex Pathway (413429); BER Complex Pathway (468377); Base Excision Repair Pathway (83043); Base Excision Repair Pathway (451)

ncbi summary :

This gene encodes poly(ADP-ribosyl)transferase-like 1 protein, which is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. Since this protein is not capable of binding DNA directly, its transferase activity may be activated by other factors such as protein-protein interaction mediated by the extensive carboxyl terminus. [provided by RefSeq, Jul 2008]

uniprot summary :

PARP4: Component of the vault ribonucleoprotein particle, at least composed of MVP, PARP4 and one or more vault RNAs (vRNAs). Binds to MVP. Associates with TEP1. Protein type: Transferase; EC 2.4.2.30. Chromosomal Location of Human Ortholog: 13q11. Cellular Component: membrane; spindle microtubule; cytoplasm; ribonucleoprotein complex; nucleus. Molecular Function: protein binding; enzyme binding; DNA binding; NAD+ ADP-ribosyltransferase activity. Biological Process: response to drug; cell death; protein amino acid ADP-ribosylation; transport; protein modification process; DNA repair; inflammatory response; response to DNA damage stimulus

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage