ELISA/assay

Human STAT5A (Signal Transducer and Activator of Transcription 5A) ELISA Kit

MBS2514167

48-Strip-Wells

410 USD

ELISA Kit

Human STAT5A (Signal Transducer and Activator of Transcription 5A) ELISA Kit

STAT5A

signal transducer and activator of transcription 5A isoform 1; Signal transducer and activator of transcription 5A; signal transducer and activator of transcription 5A; signal transducer and activator of transcription 5A

STAT5A

STAT5A; STAT5A; MGF; STAT5; STAT5

STA5A_HUMAN

Human

794

This kit recognizes natural and recombinant Human STAT5A. No significant cross-reactivity or interference between Human STAT5A and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.625-40ng/mL. Sensitivity: The minimum detectable dose of Human STAT5A is 0.375ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human STAT5A concentrations in serum, plasma and other biological fluids.

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to STAT5A. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for STAT5A and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain STAT5A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of STAT5A. You can calculate the concentration of STAT5A in the samples by comparing the OD of the samples to the standard curve.

21618342

NP_003143.2

NM_003152.3

P42229

86,949 Da

AGE/RAGE Pathway (698754); Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adipogenesis Pathway (198832); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); BDNF Signaling Pathway (712093); CD40/CD40L Signaling Pathway (138061); CXCR4-mediated Signaling Events Pathway (137910); Chronic Myeloid Leukemia Pathway (83116); Chronic Myeloid Leukemia Pathway (528)

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]

STAT5A: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 fusion protein is essential for the tumorigenesis. Induces the expression of BCL2L1/BCL-X(L) in the mouse, suggesting an antiapoptotic function of this protein. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described. Protein type: Transcription factor; Motility/polarity/chemotaxis; DNA-binding; Oncoprotein. Chromosomal Location of Human Ortholog: 17q11.2. Cellular Component: nucleoplasm; cytosol. Molecular Function: protein binding; signal transducer activity; transcription factor activity. Biological Process: lactation; succinate metabolic process; oxaloacetate metabolic process; peptidyl-tyrosine phosphorylation; positive regulation of interleukin-2 biosynthetic process; T cell differentiation in the thymus; female pregnancy; positive regulation of multicellular organism growth; positive regulation of mitotic cell cycle; positive regulation of activated T cell proliferation; fatty acid metabolic process; 2-oxoglutarate metabolic process; positive regulation of natural killer cell differentiation; sequestering of lipid; allantoin metabolic process; negative regulation of mast cell apoptosis; natural killer cell differentiation; luteinization; development of secondary male sexual characteristics; regulation of steroid metabolic process; creatinine metabolic process; T cell homeostasis; positive regulation of gamma-delta T cell differentiation; Peyer s patch development; isoleucine metabolic process; negative regulation of erythrocyte differentiation; transcription, DNA-dependent; citrate metabolic process; valine metabolic process; regulation of multicellular organism growth; development of secondary female sexual characteristics; positive regulation of natural killer cell mediated cytotoxicity; JAK-STAT cascade; creatine metabolic process; regulation of transcription from RNA polymerase II promoter; regulation of epithelial cell differentiation; positive regulation of B cell differentiation; positive regulation of transcription from RNA polymerase II promoter; taurine metabolic process; positive regulation of inflammatory response

48-Strip-Wells

410 USD

96-Strip-Wells

490