catalog number :
MBS2514139
products type :
ELISA Kit
products full name :
Rat G-CSF (Granulocyte Colony Stimulating Factor) ELISA Kit
products short name :
[G-CSF]
other names :
[granulocyte colony-stimulating factor; Granulocyte colony-stimulating factor; granulocyte colony-stimulating factor; G-CSF; colony stimulating factor 3 (granulocyte)<]
products gene name :
[G-CSF]
other gene names :
[CSF3; CSF3; GCSF; G-CSF]
uniprot entry name :
CSF3_BOVIN
specificity :
This kit recognizes natural and some recombinant Rat G-CSF. No significant crossreactivity or interference between Rat G-CSF and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 15.625-1000pg/mL. Sensitivity: Min: 9.375pg/mL; Max: 1000pg/mL
products description :
Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Rat G-CSF concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Rat G-CSF. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Rat G-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Rat G-CSF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat G-CSF. The concentration of Rat GCSF in samples can be calculated by comparing the OD of the samples with the standard curve.
ncbi acc num :
NP_776453.1
ncbi gb acc num :
NM_174028.1
ncbi mol weight :
21,431 Da
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway (84290); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response (BioCarta) Pathway (198574); Hematopoietic Cell Lineage Pathway (84317); Hematopoietic Cell Lineage Pathway (489); Jak-STAT Signaling Pathway (84316); Jak-STAT Signaling Pathway (488); Malaria Pathway (152664); Malaria Pathway (152657); PI3K-Akt Signaling Pathway (692236)
uniprot summary :
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
size5 :
10x96-Strip-Wells