catalog number :
MBS2514128
products type :
ELISA Kit
products full name :
Human REG3gamma (Regenerating Islet Derived Protein 3 Gamma) ELISA Kit
products short name :
[REG3gamma]
other names :
[regenerating islet-derived protein 3-gamma isoform 2 preproprotein; Regenerating islet-derived protein 3-gamma; regenerating islet-derived protein 3-gamma; REG-3-gamma; pancreatitis-associated protein 1B; pancreatitis-associated protein IB; reg III-gamma; regenerating gene III; regenerating islet-derived protein III-gamma; regenerating islet-derived 3 gamma; Pancreatitis-associated protein 1B; PAP-1B; Pancreatitis-associated protein IB; PAP IB; Regenerating islet-derived protein III-gamma; REG III; Reg III-gamma]
products gene name :
[REG3gamma]
other gene names :
[REG3G; REG3G; PAP1B; PAPIB; PAP IB; PAP-1B; UNQ429; LPPM429; REG III; REG-III; PAP1B; REG-3-gamma; PAP-1B; PAP IB; REG III; Reg III-gamma]
uniprot entry name :
REG3G_HUMAN
specificity :
This kit recognizes Human REG3alpha in samples. No significant cross-reactivity or interference between Human REG3alpha and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 15.63-1000pg/mL. Sensitivity: 9.38pg/mL
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human REG3alpha were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human REG3alpha were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human REG3alpha concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human REG3alpha. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human REG3alpha and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human REG3alpha, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human REG3alpha. You can calculate the concentration of Human REG3alpha in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_001256969.1
ncbi gb acc num :
NM_001270040.1
ncbi mol weight :
14,409 Da
ncbi summary :
This gene encodes a member of the regenerating islet-derived genes (REG)3 protein family. These proteins are secreted, C-type lectins with a carbohydrate recognition domain and N-terminal signal peptide. The protein encoded by this gene is an antimicrobial lectin with activity against Gram-positive bacteria. Alternative splicing results in multiple transcript variants encoding multiple isoforms. [provided by RefSeq, Nov 2014]
uniprot summary :
REG3G: Might be a stress protein involved in the control of bacterial proliferation. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 2p12. Cellular Component: cytoplasm; extracellular region. Molecular Function: carbohydrate binding. Biological Process: MyD88-dependent toll-like receptor signaling pathway; defense response to Gram-positive bacterium; negative regulation of keratinocyte differentiation; acute-phase response
size5 :
10x96-Strip-Wells