catalog number :
MBS2514119
products type :
ELISA Kit
products full name :
Human KRT10 (Keratin, Type I Cytoskeletal 10) ELISA Kit
products short name :
KRT10
other names :
keratin, type I cytoskeletal 10; Keratin, type I cytoskeletal 10; keratin, type I cytoskeletal 10; CK-10; cytokeratin 10; keratin-10; keratin 10, type I; Cytokeratin-10; CK-10; Keratin-10; K10
products gene name :
KRT10
other gene names :
KRT10; KRT10; BIE; EHK; K10; KPP; BCIE; CK10; KPP; CK-10; K10
uniprot entry name :
K1C10_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of Human KRT10. No significant cross-reactivity or interference between Human KRT10 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human KRT10 and all the analogues, therefore, cross reaction may still exist.
storage stability :
Store at 4 degree C.
other info1 :
Detection Range: 15.625--1000pg/mL. Sensitivity: 9.375pg/mL
other info2 :
Sample Type: Serum, Plasma, Biological Fluids. Method: sandwich
products description :
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of KRT10 in human serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human KRT10. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human KRT10 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human KRT10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human KRT10. You can calculate the concentration of Human KRT10 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_000412.3
ncbi gb acc num :
NM_000421.3
ncbi mol weight :
58,827 Da
ncbi pathways :
Staphylococcus Aureus Infection Pathway 172846!!Staphylococcus Aureus Infection Pathway 171867
ncbi summary :
This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq, Jul 2008]