ELISA/assay

Human SOD3 (Superoxide Dismutase 3, Extracellular) ELISA Kit

MBS2514105

48-Strip-Wells

410 USD

ELISA Kit

Human SOD3 (Superoxide Dismutase 3, Extracellular) ELISA Kit

SOD3

SOD3; Extracellular superoxide dismutase [Cu-Zn]; extracellular superoxide dismutase [Cu-Zn]; extracellular superoxide dismutase [Cu-Zn]; superoxide dismutase 3, extracellular

SOD3

SOD3; SOD3; EC-SOD; EC-SOD

SODE_HUMAN

Human

240

This kit recognizes natural and recombinant Human SOD3. No significant cross-reactivity or interference between Human SOD3 and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Competitive. Detection Range: 6.25-400ng/mL. Sensitivity: 3.75ng/mL

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human SOD3 concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with SOD3. During the reaction, SOD3 in the sample or standard competes with a fixed amount of SOD3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to SOD3. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of SOD3 in the samples is then determined by comparing the OD of the samples to the standard curve.

49456661

CAG46651.1

P08294

25,851 Da

Cellular Responses To Stress Pathway (645258); Detoxification Of Reactive Oxygen Species Pathway (1127552); Folate Metabolism Pathway (198833); Oxidative Stress Pathway (198916); Selenium Pathway (198825); Vitamin B12 Metabolism Pathway (920974); Reactive Oxygen Species Degradation Pathway (1108784); Reactive Oxygen Species Degradation (mammalian) Pathway (1108902)

This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. The product of this gene is thought to protect the brain, lungs, and other tissues from oxidative stress. The protein is secreted into the extracellular space and forms a glycosylated homotetramer that is anchored to the extracellular matrix (ECM) and cell surfaces through an interaction with heparan sulfate proteoglycan and collagen. A fraction of the protein is cleaved near the C-terminus before secretion to generate circulating tetramers that do not interact with the ECM. [provided by RefSeq, Jul 2008]

SOD3: Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen. Belongs to the Cu-Zn superoxide dismutase family. Protein type: Secreted, signal peptide; EC 1.15.1.1; Secreted; Oxidoreductase. Chromosomal Location of Human Ortholog: 4p15.2. Cellular Component: extracellular matrix; extracellular space; Golgi lumen; cytoplasm; extracellular region; trans-Golgi network; nucleus. Molecular Function: heparin binding; protein binding; copper ion binding; zinc ion binding; superoxide dismutase activity. Biological Process: response to reactive oxygen species; response to copper ion; removal of superoxide radicals; response to hypoxia

48-Strip-Wells

410 USD

96-Strip-Wells

490