Rat TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) ELISA Kit | MyBioSource MBS2514053 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) ELISA Kit

catalog :

MBS2514053

quantity :

24-Strip-Wells

price :

190 USD

product information

catalog number :

MBS2514053

products type :

ELISA Kit

products full name :

Rat TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) ELISA Kit

products short name :

[TIMP-3]

other names :

[TIMP-3; Metalloproteinase inhibitor 3; metalloproteinase inhibitor 3; MIG-5 protein; TIMP-3; protein MIG-5; tissue inhibitor of metalloproteinases 3; TIMP metallopeptidase inhibitor 3; Protein MIG-5; Tissue inhibitor of metalloproteinases 3; TIMP-3]

products gene name :

[TIMP-3]

other gene names :

[TIMP3; TIMP3; SFD; K222; K222TA2; HSMRK222; TIMP-3]

uniprot entry name :

TIMP3_HUMAN

reactivity :

Rat

sequence length :

211

specificity :

This kit recognizes natural and recombinant Rat TIMP-3. No significant cross-reactivity or interference between Rat TIMP-3 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL

other info2 :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat TIMP-3 concentrations in serum, plasma and other biological fluids.

products description :

. Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TIMP-3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TIMP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TIMP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TIMP-3. You can calculate the concentration of TIMP-3 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

998826

ncbi acc num :

AAB34532.1

uniprot acc num :

P35625

ncbi mol weight :

24,145 Da

ncbi pathways :

Angiogenesis Pathway (198772); Endochondral Ossification Pathway (198812); Matrix Metalloproteinases Pathway (198900); MicroRNAs In Cancer Pathway (852705); MicroRNAs In Cancer Pathway (852928); Oncostatin M Signaling Pathway (711361); Proteoglycans In Cancer Pathway (782000); Proteoglycans In Cancer Pathway (782054)

ncbi summary :

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy. [provided by RefSeq, Jul 2008]

uniprot summary :

TIMP3: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15. Interacts with EFEMP1. Belongs to the protease inhibitor I35 (TIMP) family. Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 22q12.3. Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; basement membrane; nucleus. Molecular Function: metalloendopeptidase inhibitor activity; protein binding; protease binding; metal ion binding. Biological Process: negative regulation of metalloenzyme activity; visual perception; response to cytokine stimulus; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis. Disease: Fundus Dystrophy, Pseudoinflammatory, Of Sorsby

size1 :

24-Strip-Wells

price1 :

190 USD

size2 :

48-Strip-Wells

price2 :

310

size3 :

96-Strip-Wells

price3 :

355

size4 :

5x96-Strip-Wells

price4 :

1435

size5 :

10x96-Strip-Wells

price5 :

2805