Porcine OPN (Osteopontin) ELISA Kit | MyBioSource MBS2514012 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Porcine OPN (Osteopontin) ELISA Kit

catalog :

MBS2514012

quantity :

24-Strip-Wells

price :

235 USD

product information

catalog number :

MBS2514012

products type :

ELISA Kit

products full name :

Porcine OPN (Osteopontin) ELISA Kit

products short name :

[OPN]

other names :

[osteopontin isoform OPN-a; Osteopontin; osteopontin; SPP1/CALPHA1 fusion; early T-lymphocyte activation 1; nephropontin; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); urinary stone protein; uropontin; secreted phosphoprotein 1; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin]

products gene name :

[OPN]

other gene names :

[SPP1; SPP1; OPN; BNSP; BSPI; ETA-1; BNSP; OPN; SPP-1]

uniprot entry name :

OSTP_HUMAN

reactivity :

Porcine

sequence length :

314

specificity :

This kit recognizes natural and recombinant Porcine OPN. No significant cross-reactivity or interference between Porcine OPN and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 62.5-4000pg/mL. Sensitivity: Min: 37.5pg/mL; Max: 4000pg/mL

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine OPN concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to OPN. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for OPN and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain OPN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of OPN. You can calculate the concentration of OPN in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

91206462

ncbi acc num :

NP_001035147.1

ncbi gb acc num :

NM_001040058.1

uniprot acc num :

P10451

ncbi mol weight :

33,843 Da

ncbi pathways :

BDNF Signaling Pathway (712093); Degradation Of The Extracellular Matrix Pathway (576263); Direct P53 Effectors Pathway (137939); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Endochondral Ossification Pathway (198812); Extracellular Matrix Organization Pathway (576262); FGF Signaling Pathway (137989); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067)

ncbi summary :

The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

uniprot summary :

osteopontin: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 4q22.1. Cellular Component: extracellular space; cell projection; perinuclear region of cytoplasm; apical part of cell; extracellular region. Molecular Function: protein binding; extracellular matrix binding; cytokine activity. Biological Process: osteoblast differentiation; neutrophil chemotaxis; extracellular matrix disassembly; extracellular matrix organization and biogenesis; response to vitamin D; biomineral formation; response to steroid hormone stimulus; negative regulation of collateral sprouting of intact axon in response to injury; positive regulation of bone resorption; decidualization; inflammatory response; cell adhesion; embryo implantation

size1 :

24-Strip-Wells

price1 :

235 USD

size2 :

48-Strip-Wells

price2 :

450

size3 :

96-Strip-Wells

price3 :

535

size4 :

5x96-Strip-Wells

price4 :

2255

size5 :

10x96-Strip-Wells

price5 :

3975