This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
ELISA/assay
product name :
Chicken PUMA (P53 Upregulated Modulator of Apoptosis) ELISA Kit
catalog :
MBS2513940
quantity :
24-Strip-Wells
price :
235 USD
product information
catalog number :
MBS2513940
products type :
ELISA Kit
products full name :
Chicken PUMA (P53 Upregulated Modulator of Apoptosis) ELISA Kit
products short name :
[PUMA]
other names :
[PUMA; Bcl-2-binding component 3; bcl-2-binding component 3; p53 up-regulated modulator of apoptosis; Bcl-2 binding component 3; p53 up-regulated modulator of apoptosis]
products gene name :
[PUMA]
other gene names :
[Bbc3; Bbc3; Puma; Puma]
uniprot entry name :
BBC3_RAT
reactivity :
Chicken
sequence length :
193
specificity :
This kit recognizes natural and recombinant Chicken PUMA. No significant cross-reactivity or interference between Chicken PUMA and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 1.563-100ng/mL. Sensitivity: Min: 0.938ng/mL; Max: 100ng/mL
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken PUMA concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PUMA. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PUMA and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PUMA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PUMA. You can calculate the concentration of PUMA in the samples by comparing the OD of the samples to the standard curve.
ncbi gi num :
27542559
ncbi acc num :
AAO16862.1
uniprot acc num :
Q80ZG6
ncbi mol weight :
20,718 Da
ncbi pathways :
Apoptosis Pathway (219785); Hippo Signaling Pathway (749790); Hippo Signaling Pathway (750388); Huntington's Disease Pathway (83492); Huntington's Disease Pathway (512); Measles Pathway (213300); Measles Pathway (213277); P53 Signaling Pathway (83447); P53 Signaling Pathway (465)
uniprot summary :
PUMA: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Interacts with MCL1 and BCL2A1. Interacts with BCL2 and BCL2L1/BCL-XL. By DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. Ubiquitously expressed. Belongs to the Bcl-2 family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Mitochondrial. Cellular Component: mitochondrion; intracellular. Molecular Function: protein binding. Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of protein homooligomerization; positive regulation of apoptosis; determination of adult life span; glial cell differentiation; reduction of endoplasmic reticulum calcium ion concentration; positive regulation of neuron apoptosis; release of sequestered calcium ion into cytosol; brain development; response to DNA damage stimulus; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; negative regulation of growth
size1 :
24-Strip-Wells
price1 :
235 USD
size2 :
48-Strip-Wells
price2 :
450
size3 :
96-Strip-Wells
price3 :
535
size4 :
5x96-Strip-Wells
price4 :
2255
size5 :
10x96-Strip-Wells
price5 :
3975
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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