catalog number :
MBS2513933
products type :
ELISA Kit
products full name :
AA (Arachidonic Acid) ELISA Kit
products short name :
[AA]
other names :
[aa, partial; Aa protein; Aa protein]
products gene name :
[AA]
uniprot entry name :
Q00378_COCIT
specificity :
This kit recognizes natural and recombinant AA. No significant cross-reactivity or interference between AA and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Competitive. Detection Range: 1.563-100ng/mL. Sensitivity: Min: 0.938ng/mL; Max: 100ng/mL
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of AA concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with AA. During the reaction, AA in the sample or standard competes with a fixed amount of AA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to AA. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of AA in the samples is then determined by comparing the OD of the samples to the standard curve.
ncbi acc num :
CAA63855.1
ncbi mol weight :
5,998 Da
size5 :
10x96-Strip-Wells