catalog number :
MBS2513798
products type :
ELISA Kit
products full name :
Human IFN-beta(Interferon Beta)ELISA Kit
products short name :
IFN-beta
other names :
interferon beta; Interferon beta; interferon beta; IFN-beta; fibroblast interferon; interferon, beta 1, fibroblast; Fibroblast interferon
products gene name :
IFN-beta
other gene names :
IFNB1; IFNB1; IFB; IFF; IFNB; IFB; IFNB; IFN-beta
uniprot entry name :
IFNB_HUMAN
specificity :
This kit recognizes natural and recombinant Human IFN-beta. No significant cross-reactivity or interference between Human IFN-beta and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: 18.75pg/mL
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human IFN-beta concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IFN-beta. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IFN-beta and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IFN-beta, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IFN-beta. You can calculate the concentration of IFN-beta in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_002167.1
ncbi gb acc num :
NM_002176.2
ncbi mol weight :
22,294 Da
ncbi pathways :
Cellular Senescence Pathway (905991); Cellular Responses To Stress Pathway (645258); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833)
uniprot summary :
IFNB1: Has antiviral, antibacterial and anticancer activities. Belongs to the alpha/beta interferon family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 9p21. Cellular Component: extracellular space; extracellular region. Molecular Function: interferon-alpha/beta receptor binding; cytokine activity. Biological Process: B cell proliferation; adaptive immune response; response to virus; cytokine and chemokine mediated signaling pathway; regulation of MHC class I biosynthetic process; natural killer cell activation; negative regulation of T cell differentiation; humoral immune response; T cell activation during immune response; cell surface receptor linked signal transduction; response to exogenous dsRNA; negative regulation of viral genome replication; B cell differentiation; positive regulation of innate immune response; defense response to bacterium; natural killer cell activation during immune response; innate immune response; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; defense response to virus; positive regulation of peptidyl-serine phosphorylation of STAT protein; B cell activation during immune response