catalog number :
MBS2513779
products type :
ELISA Kit
products full name :
Human PRDX5 (Peroxiredoxin 5) ELISA Kit
products short name :
[PRDX5]
other names :
[peroxiredoxin-5, mitochondrial isoform c; Peroxiredoxin-5, mitochondrial; peroxiredoxin-5, mitochondrial; Alu co-repressor 1; TPx type VI; antioxidant enzyme B166; epididymis secretory protein Li 55; liver tissue 2D-page spot 71B; peroxiredoxin V; peroxisomal antioxidant enzyme; thioredoxin peroxidase PMP20; thioredoxin reductase; peroxiredoxin 5; Alu corepressor 1; Antioxidant enzyme B166; AOEB166; Liver tissue 2D-page spot 71B; PLP; Peroxiredoxin V; Prx-V; Peroxisomal antioxidant enzyme; TPx type VI; Thioredoxin peroxidase PMP20; Thioredoxin reductase]
products gene name :
[PRDX5]
other gene names :
[PRDX5; PRDX5; PLP; ACR1; B166; PRXV; PMP20; PRDX6; prx-V; SBBI10; AOEB166; HEL-S-55; ACR1; AOEB166; Prx-V]
uniprot entry name :
PRDX5_HUMAN
specificity :
This kit recognizes Human PPAR-delta in samples. No significant cross-reactivity or interference between Human PPAR-delta and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.16-10ng/mL. Sensitivity: 0.1ng/mL
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human PPAR-delta were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human PPAR-delta were tested on 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human PPAR-delta concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PPAR-delta. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PPAR-delta and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PPAR-delta, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PPAR-delta. You can calculate the concentration of Human PPAR-delta in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_857635.1
ncbi gb acc num :
NM_181652.2
ncbi mol weight :
12,897 Da
ncbi pathways :
Cellular Responses To Stress Pathway (645258); Detoxification Of Reactive Oxygen Species Pathway (1127552); Peroxisome Pathway (131226); Peroxisome Pathway (131126); Selenium Pathway (198825); Thioredoxin Pathway (142228)
ncbi summary :
This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. This protein interacts with peroxisome receptor 1. The crystal structure of this protein in its reduced form has been resolved to 1.5 angstrom resolution. This gene uses alternate in-frame translation initiation sites to generate mitochondrial or peroxisomal/cytoplasmic forms. Three transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
uniprot summary :
PRDX5: Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling. Belongs to the peroxiredoxin 2 family. 2 isoforms of the human protein are produced by alternative initiation. Protein type: EC 1.11.1.15; Oxidoreductase. Chromosomal Location of Human Ortholog: 11q13. Cellular Component: extracellular space; peroxisomal matrix; intracellular membrane-bound organelle; mitochondrion; perinuclear region of cytoplasm; mitochondrial matrix; cytoplasm; peroxisome; cytoplasmic vesicle; nucleus; cytosol. Molecular Function: protein dimerization activity; antioxidant activity; peroxidase activity; peroxiredoxin activity; caspase inhibitor activity; receptor binding. Biological Process: response to reactive oxygen species; hydrogen peroxide catabolic process; negative regulation of oxidoreductase activity; negative regulation of transcription from RNA polymerase III promoter; positive regulation of collagen biosynthetic process; negative regulation of caspase activity; response to oxidative stress; inflammatory response; negative regulation of apoptosis
size5 :
10x96-Strip-Wells