Human NT-ProANP (N-Terminal Pro Atrial Natriuretic Peptide) ELISA Kit | MyBioSource MBS2513493 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human NT-ProANP (N-Terminal Pro Atrial Natriuretic Peptide) ELISA Kit

catalog :

MBS2513493

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2513493

products type :

ELISA Kit

products full name :

Human NT-ProANP (N-Terminal Pro Atrial Natriuretic Peptide) ELISA Kit

products short name :

NT-ProANP

other names :

natriuretic peptides A preproprotein; Natriuretic peptides A; natriuretic peptides A; atriopeptin; cardionatrin; natriuretic peptide precursor A; prepronatriodilatin; natriuretic peptide A; CDD-ANF; Cardiodilatin; CDD; Cardiodilatin-related peptide; CDP; PrepronatriodilatinAtrial natriuretic factor; ANFAlternative name(s):Atrial natriuretic peptide; ANP

products gene name :

NT-ProANP

other gene names :

NPPA; NPPA; ANF; ANP; PND; ATFB6; ATRST2; CDD-ANF; ANP; PND; CDD; CDP; ANF; ANP

uniprot entry name :

ANF_HUMAN

reactivity :

Human

sequence length :

151

specificity :

This kit recognizes natural and recombinant Human NT-ProANP. No significant cross-reactivity or interference between Human NT-ProANP and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 62.5-4000pg/mL. Sensitivity: The minimum detectable dose of Human NT-ProANP is 37.5pg/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).

other info2 :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human NT-ProANP concentrations in serum, plasma and other biological fluids.

products description :

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to NT-ProANP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for NT-ProANP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain NT-ProANP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of NT-ProANP. You can calculate the concentration of NT-ProANP in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

23510319

ncbi acc num :

NP_006163.1

ncbi gb acc num :

NM_006172.3

uniprot acc num :

P01160

ncbi mol weight :

16,708 Da

ncbi pathways :

Amyloids Pathway (366238); Disease Pathway (530764); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); HIF-1 Signaling Pathway (695200); MicroRNAs In Cardiomyocyte Hypertrophy Pathway (198784); YAP1- And WWTR1 (TAZ)-stimulated Gene Expression Pathway (576276)

ncbi summary :

The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. [provided by RefSeq, Sep 2009]

uniprot summary :

NPPA: Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. Defects in NPPA are the cause of familial atrial fibrillation type 6 (ATFB6). Atrial fibrillation is a common disorder of cardiac rhythm that is hereditary in a small subgroup of patients. It is characterized by disorganized atrial electrical activity, progressive deterioration of atrial electromechanical function and ineffective pumping of blood into the ventricles. It can be associated with palpitations, syncope, thromboembolic stroke, and congestive heart failure. Belongs to the natriuretic peptide family. Protein type: Secreted; Hormone; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1p36.21. Cellular Component: extracellular space; mast cell granule; perinuclear region of cytoplasm; extracellular region; nucleus. Molecular Function: neuropeptide hormone activity; peptide hormone receptor binding; receptor binding. Biological Process: cardiac muscle hypertrophy; cGMP biosynthetic process; transcription initiation from RNA polymerase II promoter; negative regulation of systemic arterial blood pressure; positive regulation of heart rate; female pregnancy; response to insulin stimulus; neuropeptide signaling pathway; receptor guanylyl cyclase signaling pathway; regulation of blood pressure; response to hypoxia; gene expression; negative regulation of cell growth; regulation of blood vessel size. Disease: Atrial Standstill 2; Atrial Fibrillation, Familial, 6

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage