Human GRbeta (Glucocorticoid Receptor Beta) ELISA Kit | MyBioSource MBS2513465 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human GRbeta (Glucocorticoid Receptor Beta) ELISA Kit

catalog :

MBS2513465

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2513465

products type :

ELISA Kit

products full name :

Human GRbeta (Glucocorticoid Receptor Beta) ELISA Kit

products short name :

GRbeta

other names :

glucocorticoid receptor isoform beta; Glucocorticoid receptor; glucocorticoid receptor; glucocorticoid nuclear receptor variant 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); Nuclear receptor subfamily 3 group C member 1

products gene name :

GRbeta

other gene names :

NR3C1; NR3C1; GR; GCR; GRL; GCCR; GCRST; GRL; GR

uniprot entry name :

GCR_HUMAN

reactivity :

Human

sequence length :

742

specificity :

This assay has high sensitivity and excellent specificity for detection of Human GRbeta. No significant cross-reactivity or interference between Human GRbeta and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human GRbeta and all the analogues, therefore, cross reaction may still exist.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 78.125--5000pg/mL. Sensitivity: 46.875pg/mL

products description :

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GRbeta in human serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GRbeta. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human GRbeta and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GRbeta, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human GRbeta. You can calculate the concentration of Human GRbeta in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

66528642

ncbi acc num :

NP_001018661.1

ncbi gb acc num :

NM_001020825.1

uniprot acc num :

P04150

ncbi mol weight :

82,904 Da

ncbi pathways :

Adipogenesis Pathway (198832); BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway (477138); Circadian Clock Pathway (187173); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Glucocorticoid Receptor Regulatory Network Pathway (138014); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462); Nuclear Receptor Transcription Pathway (105979)

ncbi summary :

This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011]

uniprot summary :

GR: transcription factor of the nuclear receptor family. Receptor for glucocorticoids (GC). Binds to glucocorticoid response elements (GRE) and modulates other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Three alternatively spliced isoforms have been described. Protein type: DNA-binding; Transcription factor; Mitochondrial; Nuclear receptor. Chromosomal Location of Human Ortholog: 5q31.3. Cellular Component: nucleoplasm; membrane; mitochondrial matrix; cytoplasm; cytosol; nucleus. Molecular Function: protein dimerization activity; protein binding; glucocorticoid receptor activity; zinc ion binding; transcription factor activity; steroid binding. Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; adrenal gland development; maternal behavior; regulation of glucocorticoid biosynthetic process; chromatin modification; signal transduction; glucocorticoid mediated signaling; glucocorticoid metabolic process; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; glucocorticoid receptor signaling pathway; regulation of gluconeogenesis; gene expression; positive regulation of transcription from RNA polymerase II promoter. Disease: Glucocorticoid Resistance, Generalized

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage