catalog number :
MBS2513430
products type :
ELISA Kit
products full name :
Rabbit MT (Melatonin) ELISA Kit
products short name :
[MT]
other names :
[Metallothionein; Metallothionein; metallothionein; MT]
products gene name :
[MT]
other gene names :
[LOC100049397; mt; MT]
uniprot entry name :
MT_ORYLA
specificity :
This kit recognizes natural and recombinant Rabbit MT. No significant cross-reactivity or interference between Rabbit MT and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Competitive. Detection Range: 31.25-2000pg/mL. Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL
other info2 :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rabbit MT concentrations in serum, plasma and other biological fluids.
products description :
Principle of the assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with MT. During the reaction, MT in the sample or standard competes with a fixed amount of MT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to MT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MT in the samples is then determined by comparing the OD of the samples to the standard curve.
ncbi mol weight :
6,007 Da
uniprot summary :
Metallothioneins have a high content of cysteine residues that bind various heavy metals.
size5 :
10x96-Strip-Wells