Mouse PDGFD (Platelet Derived Growth Factor D) ELISA Kit | MyBioSource MBS2513412 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse PDGFD (Platelet Derived Growth Factor D) ELISA Kit

catalog :

MBS2513412

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2513412

products type :

ELISA Kit

products full name :

Mouse PDGFD (Platelet Derived Growth Factor D) ELISA Kit

products short name :

PDGFD

other names :

platelet-derived growth factor D isoform 2; Platelet-derived growth factor D; platelet-derived growth factor D; PDGF-D; iris-expressed growth factor; spinal cord derived growth factor B; spinal cord-derived growth factor B; spinal cord-derived growth factor-B; platelet derived growth factor D; Iris-expressed growth factor; Spinal cord-derived growth factor B; SCDGF-B

products gene name :

PDGFD

other gene names :

PDGFD; PDGFD; IEGF; SCDGFB; MSTP036; SCDGF-B; IEGF; SCDGFB; PDGF-D; SCDGF-B; PDGFD latent form; PDGFD receptor-binding form

uniprot entry name :

PDGFD_HUMAN

reactivity :

Mouse

sequence length :

364

specificity :

This kit recognizes natural and recombinant Mouse PDGFD. No significant cross-reactivity or interference between Mouse PDGFD and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 15.625-1000pg/mL. Sensitivity: 9.375pg/mL

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse PDGFD concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PDGFD. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PDGFD and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PDGFD, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PDGFD. You can calculate the concentration of PDGFD in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

15451921

ncbi acc num :

NP_149126.1

ncbi gb acc num :

NM_033135.3

uniprot acc num :

Q9GZP0

ncbi mol weight :

42,167 Da

ncbi pathways :

Choline Metabolism In Cancer Pathway (1059539); Choline Metabolism In Cancer Pathway (1084232); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Gap Junction Pathway (83072); Gap Junction Pathway (483); Melanoma Pathway (83114); Melanoma Pathway (526); PI3K-Akt Signaling Pathway (692234)

ncbi summary :

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. Two splice variants have been identified for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

PDGFD: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Plays an important role in wound healing. Induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. Can initiate events that lead to a mesangial proliferative glomerulonephritis, including influx of monocytes and macrophages and production of extracellular matrix. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide; Oncoprotein. Chromosomal Location of Human Ortholog: 11q22.3. Cellular Component: Golgi membrane; endoplasmic reticulum lumen; extracellular region. Molecular Function: growth factor activity; platelet-derived growth factor receptor binding. Biological Process: positive regulation of cell division; multicellular organismal development; platelet-derived growth factor receptor signaling pathway; regulation of peptidyl-tyrosine phosphorylation

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage