catalog number :
MBS2513399
products type :
ELISA Kit
products full name :
Human ESM1 (Endothelial Cell Specific Molecule 1) ELISA Kit
products short name :
[ESM1]
other names :
[epithiospecifier modifier 1; GDSL esterase/lipase ESM1; epithiospecifier modifier 1; Extracellular lipase ESM1; Protein EPITHIOSPECIFIER MODIFIER 1; AtESM1]
products gene name :
[ESM1]
other gene names :
[ESM1; ESM1; epithiospecifier modifier 1; ESM1; AtESM1]
uniprot entry name :
ESM1_ARATH
specificity :
This kit recognizes natural and recombinant Human ESM1. No significant cross-reactivity or interference between Human ESM1 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 15.625-1000pg/mL. Sensitivity: Min: 9.375pg/mL; Max: 1000pg/mL
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human ESM1 concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ESM1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ESM1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ESM1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ESM1. You can calculate the concentration of ESM1 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_188037.1
ncbi gb acc num :
NM_112278.2
ncbi mol weight :
44,060 Da
ncbi summary :
A semidominant QTL which has an epistatic effect on the Epithiospecifier gene. Represses nitrile formation and favors isothiocyanate production during glucosinolate hydrolysis. The functional allele deters the insect herbivory T. ni.
uniprot summary :
Represses or inhibits nitriles production from methionine-derived and from indol-3-ylmethyl glucosinolates. Favors isothiocyanate production.
size5 :
10x96-Strip-Wells