This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
ELISA/assay
product name :
Porcine TNNI1 (Troponin I Type 1, Slow Skeletal) ELISA Kit
catalog :
MBS2513398
quantity :
24-Strip-Wells
price :
235 USD
product information
catalog number :
MBS2513398
products type :
ELISA Kit
products full name :
Porcine TNNI1 (Troponin I Type 1, Slow Skeletal) ELISA Kit
products short name :
[TNNI1]
other names :
[troponin I, slow skeletal muscle; Troponin I, slow skeletal muscle; troponin I, slow skeletal muscle; troponin I, slow-twitch isoform; troponin I type 1 (skeletal, slow); Troponin I, slow-twitch isoform]
products gene name :
[TNNI1]
other gene names :
[TNNI1; TNNI1; TNN1; SSTNI]
uniprot entry name :
TNNI1_HUMAN
reactivity :
Porcine
sequence length :
187
specificity :
This kit recognizes natural and recombinant Porcine TNNI1. No significant cross-reactivity or interference between Porcine TNNI1 and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 46.875-3000pg/mL. Sensitivity: Min: 28.125pg/mL; Max: 3000pg/mL
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine TNNI1 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TNNI1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TNNI1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TNNI1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TNNI1. You can calculate the concentration of TNNI1 in the samples by comparing the OD of the samples to the standard curve.
ncbi gi num :
56682969
ncbi acc num :
NP_003272.3
ncbi gb acc num :
NM_003281.3
uniprot acc num :
P19237
ncbi mol weight :
21,692 Da
ncbi pathways :
Muscle Contraction Pathway (106261); Striated Muscle Contraction Pathway (198903); Striated Muscle Contraction Pathway (106262)
ncbi summary :
Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes. [provided by RefSeq, Jul 2008]
uniprot summary :
TNNI1: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Belongs to the troponin I family. Protein type: Cytoskeletal; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 1q31.3. Cellular Component: troponin complex; cytosol. Molecular Function: metal ion binding; actin binding. Biological Process: regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; muscle filament sliding
size1 :
24-Strip-Wells
price1 :
235 USD
size2 :
48-Strip-Wells
price2 :
450
size3 :
96-Strip-Wells
price3 :
535
size4 :
5x96-Strip-Wells
price4 :
2255
size5 :
10x96-Strip-Wells
price5 :
3975
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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