catalog number :
MBS2513327
products type :
ELISA Kit
products full name :
3-NT (3-Nitrotyrosine) ELISA Kit
products short name :
3-NT
products gene name :
3-NT
specificity :
This kit recognizes natural and recombinant 3-NT. No significant cross-reactivity or interference between 3-NT and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Competitive. Detection Range: 1.563-100ng/mL. Sensitivity: 0.938ng/mL
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of 3-NT concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 3-NT. During the reaction, 3-NT in the sample or standard competes with a fixed amount of 3-NT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 3-NT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of 3-NT in the samples is then determined by comparing the OD of the samples to the standard curve.