ELISA/assay

Human PT (Prothrombin) ELISA Kit

MBS2513267

96 Strip Wells

520 USD

ELISA Kit

Human PT (Prothrombin) ELISA Kit

PT

prothrombin preproprotein; Prothrombin; prothrombin; prepro-coagulation factor II; prothrombin B-chain; serine protease; coagulation factor II (thrombin); Coagulation factor IICleaved into the following 4 chains:Activation peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain

PT

F2; F2; PT; THPH1; RPRGL2

THRB_HUMAN

Human

622

This assay has high sensitivity and excellent specificity for detection of Human PT. No significant cross-reactivity or interference between Human PT and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human PT and all the analogues, therefore, cross reaction may still exist.

Store at 4 degree C.

Detection Range: 9.375--600ng/mL. Sensitivity: 5.625ng/mL

Sample Type: Serum, Plasma, Biological Fluids. Method: sandwich

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PT in human serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PT. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PT and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PT. You can calculate the concentration of Human PT in the samples by comparing the OD of the samples to the standard curve.

4503635

NP_000497.1

NM_000506.3

P00734

70,037 Da

Angiopoietin Receptor Tie2-mediated Signaling Pathway 137917!!Blood Clotting Cascade Pathway 198840!!Cell Surface Interactions At The Vascular Wall Pathway 106062!!Class A/1 (Rhodopsin-like Receptors) Pathway 106357!!Common Pathway 106060!!Complement And Coagulation Cascades Pathway 198880!!Complement And Coagulation Cascades Pathway 83073!!Complement And Coagulation Cascades Pathway 484!!Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway 1127664!!Disease Pathway 530764

Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Finally, peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia. [provided by RefSeq, Nov 2014]

96 Strip Wells

520 USD