catalog number :
MBS2513255
products type :
ELISA Kit
products full name :
Human APT (Abnormal Prothrombin) ELISA Kit
products short name :
[APT]
other names :
[apt; Adenine phosphoribosyltransferase]
products gene name :
[APT]
other gene names :
[apt; ]
uniprot entry name :
A8YBM1_MICAE
specificity :
This kit recognizes natural and recombinant Human APT. No significant cross-reactivity or interference between Human APT and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human APT concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to APT. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for APT and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain APT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of APT. You can calculate the concentration of APT in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
CAO89000.1
ncbi mol weight :
18,922 Da
uniprot summary :
Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.
size5 :
10x96-Strip-Wells