ELISA/assay

Rat CTGF (Connective Tissue Growth Factor) ELISA Kit

MBS2513234

24-Strip-Wells

215 USD

ELISA Kit

Rat CTGF (Connective Tissue Growth Factor) ELISA Kit

[CTGF]

[connective tissue growth factor; Connective tissue growth factor; connective tissue growth factor; CCN family member 2; IBP-8; IGF-binding protein 8; IGFBP-8; hypertrophic chondrocyte-specific protein 24; insulin-like growth factor-binding protein 8; connective tissue growth factor; CCN family member 2; Hypertrophic chondrocyte-specific protein 24; Insulin-like growth factor-binding protein 8; IBP-8; IGF-binding protein 8; IGFBP-8]

[CTGF]

[CTGF; CTGF; CCN2; NOV2; HCS24; IGFBP8; CCN2; HCS24; IGFBP8; IBP-8; IGF-binding protein 8; IGFBP-8]

CTGF_HUMAN

Rat

349

This kit recognizes natural and recombinantRatCTGF. No significant cross-reactivity or interference between RatCTGF and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL

Intended Uses: This ELISA kit applies to the invitro quantitative determination of RatCTGFconcentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CTGF. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CTGFand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CTGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CTGF.You can calculate the concentration of CTGFin the samples by comparing the OD of the samples to the standard curve.

4503123

NP_001892.1

NM_001901.2

P29279

35,205 Da

Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Hippo Signaling Pathway (749777); Hippo Signaling Pathway (750388); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); PPARA Activates Gene Expression Pathway (530749); Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway (119544); TGF-beta Receptor Signaling Pathway (138024)

The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq, Nov 2009]

CTGF: Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. Belongs to the CCN family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide; Cell adhesion. Chromosomal Location of Human Ortholog: 6q23.1. Cellular Component: Golgi apparatus; proteinaceous extracellular matrix; extracellular space; cis-Golgi network; intracellular membrane-bound organelle; perinuclear region of cytoplasm; plasma membrane; extracellular region; cell cortex; cytosol. Molecular Function: integrin binding; heparin binding; insulin-like growth factor binding; protein binding; growth factor activity; fibronectin binding. Biological Process: response to peptide hormone stimulus; epidermis development; response to anoxia; cell-matrix adhesion; positive regulation of collagen biosynthetic process; positive regulation of caspase activity; positive regulation of JNK cascade; cellular lipid metabolic process; response to estradiol stimulus; cell-cell signaling; positive regulation of cell proliferation; positive regulation of stress fiber formation; response to wounding; tissue homeostasis; response to glucose stimulus; regulation of cell growth; angiogenesis; cell adhesion; cell differentiation; cartilage condensation; positive regulation of cell activation; integrin-mediated signaling pathway; transcription initiation from RNA polymerase II promoter; cell migration; ossification; fibroblast growth factor receptor signaling pathway; organ senescence; response to mineralocorticoid stimulus; response to amino acid stimulus; gene expression; positive regulation of protein amino acid phosphorylation; regulation of chondrocyte differentiation; positive regulation of cell differentiation; lung development

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590