catalog number :
MBS2513214
products type :
ELISA Kit
products full name :
Mouse sIgA (Secretory Immunoglobulin A) ELISA Kit
products short name :
[sIgA]
other names :
[SigA; RNA polymerase sigma factor SigA]
products gene name :
[sIgA]
other gene names :
[sigA]
uniprot entry name :
Q59965_SYNP2
specificity :
This kit recognizes Mouse SEPP1 in samples. No significant cross-reactivity or interference between Mouse SEPP1and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 125-8000pg/mL. Sensitivity: 75pg/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision withinan assay): 3 samples with low, midrange and high level MouseSEPP1were tested 20times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, midrange and high level MouseSEPP1were testedon 3 different plates, 20 replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse SEPP1 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SEPP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SEPP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SEPP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse SEPP1. You can calculate the concentration of Mouse SEPP1 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AAB41506.1
ncbi mol weight :
43,740 Da
uniprot summary :
Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.
size5 :
10x96-Strip-Wells