Human PKCeta (Protein Kinase C Eta) ELISA Kit | MyBioSource MBS2512775 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human PKCeta (Protein Kinase C Eta) ELISA Kit

catalog :

MBS2512775

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2512775

products type :

ELISA Kit

products full name :

Human PKCeta (Protein Kinase C Eta) ELISA Kit

products short name :

[PKCeta]

other names :

[protein kinase C eta type; Protein kinase C eta type; protein kinase C eta type; protein kinase C, eta; PKC-L; nPKC-eta]

products gene name :

[PKCeta]

other gene names :

[PRKCH; PRKCH; PKCL; PKC-L; PRKCL; nPKC-eta; PKCL; PRKCL]

uniprot entry name :

KPCL_HUMAN

reactivity :

Human

sequence length :

683

specificity :

This kit recognizes natural and some recombinant Human PKC?. No significant crossreactivity or interference between Human PKC? and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 3.125-200mIU/mL. Sensitivity: Min: 1.875mIU/mL; Max: 200mIU/mL

products description :

Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Human PKC? concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Human PKC?. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Human PKC? and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Human PKC?, biotinylated detection antibody and Avidin- HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PKC?. The concentration of Human PKC? in samples can be calculated by comparing the OD of the samples with the standard curve.

ncbi gi num :

28557781

ncbi acc num :

NP_006246.2

ncbi gb acc num :

NM_006255.4

uniprot acc num :

P24723

ncbi mol weight :

59,521 Da

ncbi pathways :

Calcium Regulation In The Cardiac Cell Pathway (198906); Effects Of PIP2 Hydrolysis Pathway (106044); Endothelins Pathway (137958); G Protein Signaling Pathways (198849); G Alpha (q) Signalling Events Pathway (106043); G Alpha (z) Signalling Events Pathway (119551); GPCR Downstream Signaling Pathway (119548); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Hemostasis Pathway (106028); Inflammatory Mediator Regulation Of TRP Channels Pathway (948277)

ncbi summary :

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene. [provided by RefSeq, Jul 2008]

uniprot summary :

PKCH: Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in the regulation of cell differentiation in keratinocytes and pre-B cell receptor, mediates regulation of epithelial tight junction integrity and foam cell formation, and is required for glioblastoma proliferation and apoptosis prevention in MCF-7 cells. In keratinocytes, binds and activates the tyrosine kinase FYN, which in turn blocks epidermal growth factor receptor (EGFR) signaling and leads to keratinocyte growth arrest and differentiation. Associates with the cyclin CCNE1-CDK2-p27Kip1 complex and inhibits CDK2 kinase activity, leading to RB1 dephosphorylation and thereby G1 arrest in keratinocytes. In association with RALA activates actin depolymerization, which is necessary for keratinocyte differentiation. In the pre-B cell receptor signaling, functions downstream of BLNK by up-regulating IRF4, which in turn activates L chain gene rearrangement. Regulates epithelial tight junctions (TJs) by phosphorylating occludin (OCLN) on threonine residues, which is necessary for the assembly and maintenance of TJs. In association with PLD2 and via TLR4 signaling, is involved in lipopolysaccharide (LPS)-induced RGS2 down-regulation and foam cell formation. Upon PMA stimulation, mediates glioblastoma cell proliferation by activating the mTOR pathway, the PI3K/AKT pathway and the ERK1- dependent phosphorylation of ELK1. Involved in the protection of glioblastoma cells from irradiation-induced apoptosis by preventing caspase-9 activation. In camptothecin-treated MCF-7 cells, regulates NF-kappa-B upstream signaling by activating IKBKB, and confers protection against DNA damage-induced apoptosis. Interacts with FYN and RALA. Interacts with DGKQ. Most abundant in lung, less in heart and skin. Novel PKCs (PRKCD, PRKCE, PRKCH and PRKCQ) are calcium-insensitive, but activated by diacylglycerol (DAG) and phosphatidylserine. Three specific sites; Thr-513 (activation loop of the kinase domain), Thr-656 (turn motif) and Ser-675 (hydrophobic region), need to be phosphorylated for its full activation. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; EC 2.7.11.13; Kinase, protein; AGC group; PKC family; Eta subfamily. Chromosomal Location of Human Ortholog: 14q23.1. Cellular Component: cytoplasm; plasma membrane; intercellular junction; cytosol. Molecular Function: calcium-independent protein kinase C activity; enzyme binding; protein kinase C activity; metal ion binding; Ral GTPase binding; ATP binding. Biological Process: platelet activation; positive regulation of B cell receptor signaling pathway; blood coagulation; signal transduction; protein amino acid phosphorylation; positive regulation of keratinocyte differentiation; activation of NF-kappaB transcription factor. Disease: Stroke, Ischemic

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590