Human UCHL1 (Ubiquitin Carboxyl Terminal Hydrolase L1) ELISA Kit | MyBioSource MBS2512760 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human UCHL1 (Ubiquitin Carboxyl Terminal Hydrolase L1) ELISA Kit

catalog :

MBS2512760

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2512760

products type :

ELISA Kit

products full name :

Human UCHL1 (Ubiquitin Carboxyl Terminal Hydrolase L1) ELISA Kit

products short name :

UCHL1

other names :

ubiquitin carboxyl-terminal hydrolase isozyme L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin carboxyl-terminal hydrolase isozyme L1; epididymis luminal protein 117; neuron cytoplasmic protein 9.5; ubiquitin C-terminal hydrolase; ubiquitin thioesterase L1; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1

products gene name :

UCHL1

other gene names :

UCHL1; UCHL1; NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5; UCH-L1; PGP9.5

uniprot entry name :

UCHL1_HUMAN

reactivity :

Human

sequence length :

223

specificity :

This kit recognizes natural and recombinant Human UCHL1. No significant cross-reactivity or interference between Human UCHL1 and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 78.125-5000pg/mL. Sensitivity: The minimum detectable dose of Human UCHL1 is 46.875pg/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).

other info2 :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human UCHL1 concentrations in serum, plasma and other biological fluids.

products description :

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to UCHL1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for UCHL1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain UCHL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of UCHL1. You can calculate the concentration of UCHL1 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

21361091

ncbi acc num :

NP_004172.2

ncbi gb acc num :

NM_004181.4

uniprot acc num :

P09936

ncbi mol weight :

24,824 Da

ncbi pathways :

Alpha-synuclein Signaling Pathway (137913); Parkinson's Disease Pathway (83098); Parkinsons Disease Pathway (705377); Proteasome Degradation Pathway (198786)

ncbi summary :

The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]

uniprot summary :

UCHL1: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. Monomer. Homodimer. Interacts with SNCA. Interacts with COPS5. Found in neuronal cell bodies and processes throughout the neocortex. Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. Belongs to the peptidase C12 family. Protein type: EC 3.4.19.12; Cell development/differentiation; Ligase; Ubiquitin conjugating system; Protease. Chromosomal Location of Human Ortholog: 4p14. Cellular Component: nucleoplasm; endoplasmic reticulum membrane; cell soma; axon; cytoplasm; plasma membrane; cytosol. Molecular Function: ubiquitin binding; protein binding; omega peptidase activity; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; alpha-2A adrenergic receptor binding; ligase activity. Biological Process: cell proliferation; proteasomal ubiquitin-dependent protein catabolic process; negative regulation of MAP kinase activity; axon transport of mitochondrion; axon target recognition; protein deubiquitination; eating behavior; neuromuscular process; sensory perception of pain; muscle fiber development; adult walking behavior. Disease: Neurodegeneration With Optic Atrophy, Childhood-onset; Parkinson Disease 5, Autosomal Dominant

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage