Human BECN1 (Beclin 1)ELISA Kit | MyBioSource MBS2512714 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human BECN1 (Beclin 1)ELISA Kit

catalog :

MBS2512714

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2512714

products type :

ELISA Kit

products full name :

Human BECN1 (Beclin 1)ELISA Kit

products short name :

[BECN1]

other names :

[beclin-1; Beclin-1; beclin-1; ATG6 autophagy related 6 homolog; beclin 1 (coiled-coil, moesin-like BCL2 interacting protein); beclin 1 (coiled-coil, moesin-like BCL2-interacting protein); coiled-coil myosin-like BCL2-interacting protein; beclin 1, autophagy related; Coiled-coil myosin-like BCL2-interacting protein; Protein GT197]

products gene name :

[BECN1]

other gene names :

[BECN1; BECN1; ATG6; VPS30; beclin1; GT197]

uniprot entry name :

BECN1_HUMAN

reactivity :

Human

sequence length :

450

specificity :

This kit recognizes natural and some recombinant Human BECN1. No significant crossreactivity or interference between Human BECN1 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL

products description :

Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Human BECN1 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Human BECN1. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Human BECN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Human BECN1, biotinylated detection antibody and Avidin- HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human BECN1. The concentration of Human BECN1 in samples can be calculated by comparing the OD of the samples with the standard curve.

ncbi gi num :

4502395

ncbi acc num :

NP_003757.1

ncbi gb acc num :

NM_003766.3

uniprot acc num :

Q14457

ncbi mol weight :

51,896 Da

ncbi pathways :

Regulation Of Autophagy Pathway (83058); Regulation Of Autophagy Pathway (468); Senescence And Autophagy Pathway (198780)

ncbi summary :

Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).[supplied by OMIM, Jul 2010]

uniprot summary :

beclin 1: an essential autophagy protein that has been linked to multiple processes including tumor suppression, protection against some cardiac and neurological degenerative diseases, and lifespan extension. A ubiquitous protein of the beclin family. Is part of the class III PI3K complex that induces autophagy. Expressed at elevated levels in dendrites and cell bodies of cerebellar Purkinje cells. Interacts with the anti-apoptotic protein Bcl-2, which inhibits autophagy when it is present in the endoplasmic reticulum. The dissociation of ATG6 from Bcl-2 is essential for its autophagic activity. Interacts with PITC and GluR-delta2. Probably forms a complex with AMBRA1 and PIK3C3. Plays a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus. Protein type: Adaptor/scaffold; Membrane protein, peripheral; Autophagy. Chromosomal Location of Human Ortholog: 17q21. Cellular Component: extrinsic to membrane; mitochondrion; endoplasmic reticulum; cytoplasmic membrane-bound vesicle; dendrite; trans-Golgi network; phagocytic vesicle; nucleus; endosome. Molecular Function: protein binding; phosphoinositide 3-kinase binding. Biological Process: response to drug; viral reproduction; late endosome to vacuole transport; cytokinesis; CVT pathway; cellular response to nitrogen starvation; negative regulation of cell proliferation; response to vitamin E; lysosome organization and biogenesis; regulation of catalytic activity; beta-amyloid metabolic process; mitochondrion degradation; response to hypoxia; neuron development; cellular defense response; defense response to virus; positive regulation of macroautophagy; positive regulation of autophagy; autophagic vacuole formation; negative regulation of apoptosis

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590