ELISA/assay

Porcine BPI (Bactericidal/Permeability Increasing Protein) ELISA Kit

MBS2512693

24-Strip-Wells

235 USD

ELISA Kit

Porcine BPI (Bactericidal/Permeability Increasing Protein) ELISA Kit

[BPI]

[bactericidal permeability-increasing protein; Bactericidal permeability-increasing protein; bactericidal permeability-increasing protein; BPI fold containing family D, member 1; CAP 57; recombinant BPI holoprotein, rBPI; bactericidal/permeability-increasing protein; CAP 57]

[BPI]

[BPI; BPI; rBPI; BPIFD1; BPI]

BPI_HUMAN

Porcine

487

This kit recognizes natural and recombinant Porcine BPI. No significant cross-reactivity or interference between Porcine BPI and analogues was observed.

Store at 4 degree C.

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL

Intended Uses This ELISA kit applies to the in vitro quantitative determination of Porcine BPI concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to BPI. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for BPI and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BPI, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of BPI. You can calculate the concentration of BPI in the samples by comparing the OD of the samples to the standard curve.

157276599

NP_001716.2

NM_001725.2

P17213

53,900 Da

This gene encodes a lipopolysaccharide binding protein. It is associated with human neutrophil granules and has antimicrobial activity against gram-negative organisms. [provided by RefSeq, Nov 2014]

BPI: The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. Protein type: Secreted, signal peptide; Secreted; Cell surface. Chromosomal Location of Human Ortholog: 20q11.23. Cellular Component: integral to plasma membrane; cytoplasm. Molecular Function: lipopolysaccharide binding. Biological Process: negative regulation of interleukin-6 production; negative regulation of interleukin-8 production; immune response; negative regulation of tumor necrosis factor production; negative regulation of macrophage activation; defense response to Gram-negative bacterium

24-Strip-Wells

235 USD

48-Strip-Wells

450

96-Strip-Wells

535

5x96-Strip-Wells

2255

10x96-Strip-Wells

3975