Mouse CFD (Complement Factor D) ELISA Kit | MyBioSource MBS2512656 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse CFD (Complement Factor D) ELISA Kit

catalog :

MBS2512656

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2512656

products type :

ELISA Kit

products full name :

Mouse CFD (Complement Factor D) ELISA Kit

products short name :

[CFD]

other names :

[complement factor D preproprotein; Complement factor D; complement factor D; C3 convertase activator; D component of complement (adipsin); complement factor D preproprotein; properdin factor D; complement factor D (adipsin); Adipsin; C3 convertase activator; Properdin factor D]

products gene name :

[CFD]

other gene names :

[CFD; CFD; DF; ADN; PFD; ADIPSIN; DF; PFD]

uniprot entry name :

CFAD_HUMAN

reactivity :

Mouse

sequence length :

253

specificity :

This kit recognizes natural and recombinantMouseCFD. No significant cross-reactivity or interference between MouseCFD and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 46.875-3000pg/mL. Sensitivity: Min: 28.125pg/mL; Max: 3000pg/mL

products description :

Intended Uses: This ELISA kit applies to the invitro quantitative determination of MouseCFDconcentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CFD. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CFDand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CFD, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CFD.You can calculate the concentration of CFDin the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

42544239

ncbi acc num :

NP_001919.2

ncbi gb acc num :

NM_001928.2

uniprot acc num :

P00746

ncbi mol weight :

27,033 Da

ncbi pathways :

Adipogenesis Pathway (198832); Alternative Complement Activation Pathway (106410); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (106405); Hemostasis Pathway (106028); Immune System Pathway (106386); Initial Triggering Of Complement Pathway (106406); Innate Immune System Pathway (106387); Platelet Activation, Signaling And Aggregation Pathway (106034)

ncbi summary :

The protein encoded by this gene is a member of the trypsin family of peptidases. The encoded protein is a component of the alternative complement pathway best known for its role in humoral suppression of infectious agents. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. [provided by RefSeq, Jul 2008]

uniprot summary :

CFD: Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. Defects in CFD are the cause of complement factor D deficiency (CFDD). CFDD is an immunologic disorder characterized by increased susceptibility to bacterial infections, particularly Neisseria infections, due to a defect in the alternative complement pathway. Belongs to the peptidase S1 family. Protein type: EC 3.4.21.46; Secreted; Secreted, signal peptide; Protease. Chromosomal Location of Human Ortholog: 19p13.3. Cellular Component: extracellular space; extracellular region. Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity. Biological Process: platelet activation; platelet degranulation; complement activation, alternative pathway; innate immune response; proteolysis; blood coagulation; complement activation. Disease: Complement Factor D Deficiency

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590