Human MYH10 (Myosin Heavy Chain 10, Non Muscle) ELISA Kit | MyBioSource MBS2512654 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human MYH10 (Myosin Heavy Chain 10, Non Muscle) ELISA Kit

catalog :

MBS2512654

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2512654

products type :

ELISA Kit

products full name :

Human MYH10 (Myosin Heavy Chain 10, Non Muscle) ELISA Kit

products short name :

[MYH10]

other names :

[myosin-10 isoform 1; Myosin-10; myosin-10; cellular myosin heavy chain, type B; myosin heavy chain, nonmuscle type B; myosin, heavy polypeptide 10, non-muscle; nonmuscle myosin II heavy chain-B; nonmuscle myosin heavy chain IIB; myosin, heavy chain 10, non-muscle; Cellular myosin heavy chain, type B; Myosin heavy chain 10; Myosin heavy chain, non-muscle IIb; Non-muscle myosin heavy chain B; NMMHC-B; Non-muscle myosin heavy chain IIb; NMMHC II-b; NMMHC-IIB]

products gene name :

[MYH10]

other gene names :

[MYH10; MYH10; NMMHCB; NMMHC-IIB; NMMHC-B; NMMHC II-b; NMMHC-IIB]

uniprot entry name :

MYH10_HUMAN

reactivity :

Human

sequence length :

2007

specificity :

This kit recognizes natural and some recombinant Human MYH10. No significant crossreactivity or interference between Human MYH10 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL

products description :

Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Human MYH10 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Human MYH10. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Human MYH10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Human MYH10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MYH10. The concentration of Human MYH10 in samples can be calculated by comparing the OD of the samples with the standard curve.

ncbi gi num :

365192532

ncbi acc num :

NP_001242941.1

ncbi gb acc num :

NM_001256012.1

uniprot acc num :

P35580

ncbi mol weight :

230,030 Da

ncbi pathways :

Axon Guidance Pathway (105688); Developmental Biology Pathway (477129); EPH-Ephrin Signaling Pathway (1127691); EPHA-mediated Growth Cone Collapse Pathway (1127692); Regulation Of Actin Cytoskeleton Pathway (198874); Regulation Of Actin Cytoskeleton Pathway (83089); Regulation Of Actin Cytoskeleton Pathway (500); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149); Sema4D In Semaphorin Signaling Pathway (119520)

ncbi summary :

This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-10 (MYO10). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene have been associated with May-Hegglin anomaly and developmental defects in brain and heart. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

uniprot summary :

MYH10: Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. Myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Interacts with PLEKHG6. Interacts with ECM29. Interacts with KIF26B. Interacts with LARP6. Isoform 1 is expressed in cerebellum and spinal chord. Isoform 2 is expressed in cerebrum and retina. Isoform 3 is expressed in the cerebrum and to a much lower extent in cerebellum. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Motor; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 17p13. Cellular Component: mitochondrion; myosin II complex; dendritic spine; actomyosin; cell cortex; actin cytoskeleton; growth cone; cell soma; lamellipodium; axon; cytoplasm; stress fiber; plasma membrane; spindle; midbody; nucleus; neuromuscular junction; myosin complex; cleavage furrow. Molecular Function: actin filament binding; microfilament motor activity; calmodulin binding; protein binding; actin-dependent ATPase activity; actin binding; ADP binding; ATP binding. Biological Process: adult heart development; axon guidance; exocytosis; actin filament-based movement; in utero embryonic development; metabolic process; fourth ventricle development; actomyosin structure organization and biogenesis; ventricular cardiac muscle cell development; neuron migration; cerebellar Purkinje cell layer development; cardiac myofibril assembly; third ventricle development; substrate-bound cell migration, cell extension; regulation of cell shape; cell proliferation; cytokinesis after mitosis; retina development in camera-type eye; plasma membrane repair; ephrin receptor signaling pathway; cell adhesion; neuromuscular process controlling balance; nuclear migration; lateral ventricle development

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590