ELISA/assay

Human PYY (Peptide YY) ELISA Kit

MBS2512606

24-Strip-Wells

215 USD

ELISA Kit

Human PYY (Peptide YY) ELISA Kit

[PYY]

[PYY; Peptide YY; peptide YY; peptide tyrosine tyrosine; prepro-PYY; peptide YY; PYY-I; Peptide tyrosine tyrosine; PYY-II]

[PYY]

[PYY; PYY; PYY1; PYY-I; PYY]

PYY_HUMAN

Human

90

This kit recognizes natural and recombinant Human PYY. No significant cross-reactivity or interference between Human PYY and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human PYY concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PYY. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PYY and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PYY, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PYY. You can calculate the concentration of PYY in the samples by comparing the OD of the samples to the standard curve.

49457254

CAG46926.1

P10082

10,360 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Metabolic Disorders Of Biological Oxidation Enzymes Pathway (1127637); Peptide Ligand-binding Receptors Pathway (106358); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)

The protein encoded by this gene is proteolytically processed to release a peptide that inhibits pancreatic secretion and mobility in the gut. Rare variations in this gene could increase susceptibility to obesity. [provided by RefSeq, Jul 2010]

PYY: This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility. Belongs to the NPY family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 17q21.1. Cellular Component: extracellular space; extracellular region. Molecular Function: neuropeptide hormone activity; protein binding; G-protein-coupled receptor binding; hormone activity. Biological Process: G-protein coupled receptor protein signaling pathway; cell proliferation; cell-cell signaling; neuropeptide signaling pathway; eating behavior; regulation of appetite; digestion; cytoskeleton organization and biogenesis; feeding behavior; cell motility. Disease: Obesity

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590