ELISA/assay

Rat PRL (Prolactin) ELISA Kit

MBS2512489

48-Strip-Wells

410 USD

ELISA Kit

Rat PRL (Prolactin) ELISA Kit

PRL

Prolactin; Prolactin; prolactin; decidual prolactin; prolactin

PRL

PRL; PRL; PRL

PRL_HUMAN

Rat

227

This kit recognizes natural and recombinant Rat PRL. No significant cross-reactivity or interference between Rat PRL and analogues was observed.

Store at 4 degree C.

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.781-50ng/mL. Sensitivity: 0.469ng/mL

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat PRL concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PRL. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PRL and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PRL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of PRL. You can calculate the concentration of PRL in the samples by comparing the OD of the samples to the standard curve.

130930

P01236.1

P01236

25,876 Da

Amyloids Pathway (366238); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Disease Pathway (530764); ErbB4 Signaling Events Pathway (137991); Glucocorticoid Receptor Regulatory Network Pathway (138014); Growth Hormone Receptor Signaling Pathway (477128); Immune System Pathway (106386); Jak-STAT Signaling Pathway (83077)

This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]

prolactin: Prolactin acts primarily on the mammary gland by promoting lactation. Belongs to the somatotropin/prolactin family. Protein type: Cytokine; Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 6p22.3. Cellular Component: extracellular region. Molecular Function: protein binding; prolactin receptor binding; hormone activity. Biological Process: lactation; cell proliferation; cell surface receptor linked signal transduction; positive regulation of JAK-STAT cascade; regulation of multicellular organism growth; female pregnancy

48-Strip-Wells

410 USD

96-Strip-Wells

490