catalog number :
MBS2512481
products type :
ELISA Kit
products full name :
Human RANkappaL (Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit
products short name :
RANkappaL
products gene name :
RANkappaL
specificity :
This assay has high sensitivity and excellent specificity for detection of Human RANkappaL. No significant cross-reactivity or interference between Human RANkappaL and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human RANkappaL and all the analogues, therefore, cross reaction may still exist.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156--10ng/mL. Sensitivity: 0.094ng/mL
products description :
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of RANkappaL in human serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RANkappaL. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human RANkappaL and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RANkappaL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human RANkappaL. You can calculate the concentration of Human RANkappaL in the samples by comparing the OD of the samples to the standard curve.