Rat CA9 (Carbonic Anhydrase IX) ELISA Kit | MyBioSource MBS2512480 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat CA9 (Carbonic Anhydrase IX) ELISA Kit

catalog :

MBS2512480

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2512480

products type :

ELISA Kit

products full name :

Rat CA9 (Carbonic Anhydrase IX) ELISA Kit

products short name :

[CA9]

other names :

[carbonic anhydrase 9; Carbonic anhydrase 9; carbonic anhydrase 9; CA-IX; P54/58N; RCC-associated antigen G250; RCC-associated protein G250; carbonate dehydratase IX; carbonic dehydratase; membrane antigen MN; pMW1; renal cell carcinoma-associated antigen G250; carbonic anhydrase IX; Carbonate dehydratase IX; Carbonic anhydrase IX; CA-IX; CAIX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; RCC-associated antigen G250; pMW1]

products gene name :

[CA9]

other gene names :

[CA9; CA9; MN; CAIX; G250; MN; CA-IX; CAIX; RCC-associated antigen G250]

uniprot entry name :

CAH9_HUMAN

reactivity :

Rat

sequence length :

459

specificity :

This kit recognizes natural and some recombinant Rat CA9. No significant cross-reactivity or interference between Rat CA9 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL

products description :

Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Rat CA9 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Rat CA9. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Rat CA9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Rat CA9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CA9. The concentration of Rat CA9 in samples can be calculated by comparing the OD of the samples with the standard curve.

ncbi gi num :

169636420

ncbi acc num :

NP_001207.2

ncbi gb acc num :

NM_001216.2

uniprot acc num :

Q16790

ncbi mol weight :

49,698 Da

ncbi pathways :

Cellular Response To Hypoxia Pathway (645259); Cellular Responses To Stress Pathway (645258); HIF-1-alpha Transcription Factor Network Pathway (138045); Metabolism Pathway (477135); Nitrogen Metabolism Pathway (83025); Nitrogen Metabolism Pathway (416); Regulation Of Hypoxia-inducible Factor (HIF) By Oxygen Pathway (645260); Regulation Of Gene Expression By Hypoxia-inducible Factor Pathway (645263); Reversible Hydration Of Carbon Dioxide Pathway (645352)

ncbi summary :

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]

uniprot summary :

CA9: Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. Forms oligomers linked by disulfide bonds. By hypoxia. Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. Inhibited by coumarins, saccharin, sulfonamide derivatives such as acetazolamide (AZA) and Foscarnet (phosphonoformate trisodium salt). Belongs to the alpha-carbonic anhydrase family. Protein type: Energy Metabolism - nitrogen; Membrane protein, integral; Nucleolus; EC 4.2.1.1; Lyase. Chromosomal Location of Human Ortholog: 9p13.3. Cellular Component: microvillus membrane; basolateral plasma membrane; integral to membrane; nucleolus; plasma membrane. Molecular Function: carbonate dehydratase activity; zinc ion binding. Biological Process: response to drug; secretion; bicarbonate transport; morphogenesis of an epithelium; response to testosterone stimulus; one-carbon compound metabolic process

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590